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dc.contributor.author
Citores, Lucía
dc.contributor.author
Iglesias, Rosario
dc.contributor.author
Gay, Claudia Carolina
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dc.contributor.author
Ferreras, José Miguel
dc.date.available
2018-04-06T17:25:51Z
dc.date.issued
2016-02
dc.identifier.citation
Citores, Lucía; Iglesias, Rosario; Gay, Claudia Carolina; Ferreras, José Miguel; Antifungal activity of the ribosome-inactivating protein BE27 from sugar beet (Beta vulgaris L.) against the green mould Penicillium digitatum; Wiley-Blackwell; Molecular Plant Pathology; 17; 2; 2-2016; 261-271
dc.identifier.issn
1464-6722
dc.identifier.uri
http://hdl.handle.net/11336/41157
dc.description.abstract
The ribosome-inactivating protein BE27 from sugar beet (Betavulgaris L.) leaves is an apoplastic protein induced by signallingcompounds, such as hydrogen peroxide and salicylic acid, whichhas been reported to be involved in defence against viruses. Here,we report that, at a concentration much lower than that present inthe apoplast, BE27 displays antifungal activity against the greenmould Penicillium digitatum, a necrotrophic fungus that colonizeswounds and grows in the inter- and intracellular spaces of thetissues of several edible plants. BE27 is able to enter into thecytosol and kill fungal cells, thus arresting the growth of thefungus. The mechanism of action seems to involve ribosomal RNA(rRNA) N-glycosylase activity on the sarcin?ricin loop of the majorrRNA which inactivates irreversibly the fungal ribosomes, thusinhibiting protein synthesis. We compared the C-terminus of theBE27 structure with antifungal plant defensins and hypothesizethat a structural motif composed of an α-helix and a β-hairpin,similar to the γ-core motif of defensins, might contribute to thespecific interaction with the fungal plasma membranes, allowingthe protein to enter into the cell.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-Blackwell
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Apoplast
dc.subject
Defensin
dc.subject
Green Mould
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Plant Defence
dc.subject
Pokeweed Antiviral Protein
dc.subject
Polynucleotide:Adenosine Glycosylase
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Antifungal activity of the ribosome-inactivating protein BE27 from sugar beet (Beta vulgaris L.) against the green mould Penicillium digitatum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-28T14:14:19Z
dc.identifier.eissn
1364-3703
dc.journal.volume
17
dc.journal.number
2
dc.journal.pagination
261-271
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Citores, Lucía. Universidad de Valladolid. Facultad de Ciencias; España
dc.description.fil
Fil: Iglesias, Rosario. Universidad de Valladolid. Facultad de Ciencias; España
dc.description.fil
Fil: Gay, Claudia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
dc.description.fil
Fil: Ferreras, José Miguel. Universidad de Valladolid. Facultad de Ciencias; España
dc.journal.title
Molecular Plant Pathology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mpp.12278
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12278
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