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dc.contributor.author
Pallarola, Diego Andres  
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Bochen, Alexander  
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Guglielmotti, Victoria  
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Oswald, Tabea A.  
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Kessler, Horst  
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Spatz, Joachim P.  
dc.date.available
2018-04-06T17:21:49Z  
dc.date.issued
2017-09  
dc.identifier.citation
Pallarola, Diego Andres; Bochen, Alexander; Guglielmotti, Victoria; Oswald, Tabea A.; Kessler, Horst; et al.; Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions; American Chemical Society; Analytical Chemistry; 89; 18; 9-2017; 10054-10062  
dc.identifier.issn
0003-2700  
dc.identifier.uri
http://hdl.handle.net/11336/41153  
dc.description.abstract
The formation of new types of sensitive conductive surfaces for the detection and transduction of cell–extracellular matrix recognition events in a real time, label-free manner is of great interest in the field of biomedical research. To study molecularly defined cell functions, biologically inspired materials that mimic the nanoscale order of extracellular matrix protein fibers and yield suitable electrical charge transfer characteristics are highly desired. Our strategy to achieve this goal is based on the spatial self-organization of patches of cell-adhesive molecules onto a gold-nanoparticle-patterned indium tin oxide electrode. Fibroblast adhesion response to selective ligands for integrins α5β1 and αvβ3, which are both relevant in cancer progression, is investigated by simultaneous electrochemical impedance spectroscopy and optical microscopy. Adhesive cells on α5β1-selective nanopatterns showed enhanced membrane dynamics and tighter binding, compared with cells on αvβ3-selective nanopatterns. The surface of the electrode exhibits high sensitivity to small changes in surface properties, because of the constitution of specific cell-surface interactions. Moreover, such sensitivity enables differentiation between cell types. This is exemplified by analyzing distinct features in the electrochemical readout of MCF-7 breast cancer cells versus MCF-10A mammary epithelial cells, when subjected to individual adhesive nanopatches.  
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application/pdf  
dc.language.iso
eng  
dc.publisher
American Chemical Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Electrochemical Impedance Sensor  
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Cell Adhesion  
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Integrins  
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Gold Nanopatterns  
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Transparent Microelectrodes  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
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Otras Biotecnologías de la Salud  
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Biotecnología de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
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info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-04-06T13:44:52Z  
dc.journal.volume
89  
dc.journal.number
18  
dc.journal.pagination
10054-10062  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Pallarola, Diego Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
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Fil: Bochen, Alexander. Technische Universitat Munchen; Alemania  
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Fil: Guglielmotti, Victoria. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina  
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Fil: Oswald, Tabea A.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania  
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Fil: Kessler, Horst. Technische Universitat Munchen; Alemania  
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Fil: Spatz, Joachim P.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania  
dc.journal.title
Analytical Chemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.analchem.7b02743  
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info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.7b02743