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dc.contributor.author
Pellegrotti, Jesica Vanesa
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Acuña, Guillermo
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Puchkova, Anastasiya
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Holzmeister, Phil
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Gietl, Andreas
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Lalkens, Birka
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Stefani, Fernando Daniel
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Tinnefeld, Philip
dc.date.available
2016-02-10T15:51:25Z
dc.date.issued
2014-04
dc.identifier.citation
Pellegrotti, Jesica Vanesa; Acuña, Guillermo; Puchkova, Anastasiya; Holzmeister, Phil; Gietl, Andreas; et al.; Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids; American Chemical Society; Nano Letters; 14; 5; 4-2014; 2831-2836
dc.identifier.issn
1530-6984
dc.identifier.uri
http://hdl.handle.net/11336/4098
dc.description.abstract
The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles? size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Dna Self-Assembly
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Single Molecule
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Photobleaching
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Plasmonics
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Óptica
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Ciencias Físicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids
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info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
14
dc.journal.number
5
dc.journal.pagination
2831-2836
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Pellegrotti, Jesica Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
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Fil: Acuña, Guillermo. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
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Fil: Puchkova, Anastasiya. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
dc.description.fil
Fil: Holzmeister, Phil. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
dc.description.fil
Fil: Gietl, Andreas. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
dc.description.fil
Fil: Lalkens, Birka. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
dc.description.fil
Fil: Stefani, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
dc.description.fil
Fil: Tinnefeld, Philip. Technische Universität Braunschweig. Institute for Physical and Theoretical Chemistry. NanoBioSciences Group; Alemania
dc.journal.title
Nano Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/nl500841n
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1021/nl500841n
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info:eu-repo/semantics/altIdentifier/issn/1530-6984
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