A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: performance in vitro and in live cells

We report the synthesis of a near-infrared (NIR) fluorescent pHprobe with a remarkable Stokes shift reported (∼ 135 nm) basedon a tricarbocyanine (Cy-PIP). The fluorescent molecule wasanchored to SiO2 nanoparticles (Cy-PIP@SiO2) and is capable ofmonitoring pH changes within the physiological range (pH 6-8).The Cy-PIP@SiO2 nanoparticles were succesfully internalized byHeLa cells as shown by fluorescence confocal microscopy, whileflow cytometry revealed pH fluctuations during the endocytic pathway.

Palabras clave: Nanoparticles , Cyanines , Fluorescence , Ph

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/40911

DOI: http://dx.doi.org/10.1039/C7TB00622E

URL: http://pubs.rsc.org/en/Content/ArticleLanding/2017/TB/C7TB00622E

Colecciones

Articulos(CIHIDECAR)
Articulos de CENTRO DE INVESTIGACIONES EN HIDRATOS DE CARBONO

Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL

Citación

Toum Terrones, Yamili; Coluccio Leskow, Federico; Bordoni, Andrea Veronica; Acebedo, Sofía Lorena; Spagnuolo, Carla Cecilia; et al.; A silica supported tricarbocyanine based pH nanosensor with a large Stokes shift and a near infrared fluorescence response: performance in vitro and in live cells; Royal Society of Chemistry; Journal of Materials Chemistry B; 5; 5-2017; 4031-4034

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