Artículo
Tonoplast ( Bv TIP1;2) and plasma membrane ( Bv PIP2;1) aquaporins show different mechanosensitive properties
Goldman, Ramiro Pablo; Jozefkowicz, Cintia
; Canessa Fortuna, Agustina; Sutka, Moira Romina
; Alleva, Karina Edith
; Ozu, Marcelo
Fecha de publicación:
06/2017
Editorial:
Elsevier Science
Revista:
FEBS Letters
ISSN:
0014-5793
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Previous works proposed that aquaporins behave as mechanosensitive channels. However, principal issues about mechanosensitivity of aquaporins are not known. In this work, we characterized the mechanosensitive properties of the water channels BvTIP1;2 (TIP1) and BvPIP2;1 (PIP2) from red beet (Beta vulgaris). We simultaneously measured the mechanical behavior and the water transport rates during the osmotic response of emptied-out oocytes expressing TIP1 or PIP2. Our results indicate that TIP1 is a mechanosensitive aquaporin, whereas PIP2 is not. We found that a single exponential function between the osmotic permeability coefficient and the volumetric elastic modulus governs the mechanosensitivity of TIP1. Finally, homology modeling analysis indicates that putative residues involved in mechanosensitivity show different quantity and distribution in TIP1 and PIP2.
Palabras clave:
Aquaporins
,
Mechanosensitivity
,
Membrane Tension
,
Water Permeability
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBBEA)
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos(IFIBIO HOUSSAY)
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos de INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Goldman, Ramiro Pablo; Jozefkowicz, Cintia; Canessa Fortuna, Agustina; Sutka, Moira Romina; Alleva, Karina Edith; et al.; Tonoplast ( Bv TIP1;2) and plasma membrane ( Bv PIP2;1) aquaporins show different mechanosensitive properties; Elsevier Science; FEBS Letters; 591; 11; 6-2017; 1555-1565
Compartir
Altmétricas
19 readers on Mendeley