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Artículo

Subtelomeric I-SceI-mediated double-strand breaks are repaired by homologous recombination in Trypanosoma cruzi

Chiurillo, Miguel A.; Barros, Roberto R. Moraes; Souza, Renata T.; Marini, Marjorie M.; Antonio, Cristiane R.; Cortez, Danielle R.; Curto, Maria de Los AngelesIcon ; Lorenzi, Hernán A.; Schijman, Alejandro GabrielIcon ; Ramirez, José L.; da Silveira, José F.
Fecha de publicación: 12/2016
Editorial: Frontiers Research Foundation
Revista: Frontiers in Microbiology
e-ISSN: 1664-302X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Trypanosoma cruzi chromosome ends are enriched in surface protein genes and pseudogenes (e.g., trans-sialidases) surrounded by repetitive sequences. It has been proposed that the extensive sequence variability among members of these protein families could play a role in parasite infectivity and evasion of host immune response. In previous reports we showed evidence suggesting that sequences located in these regions are subjected to recombination. To support this hypothesis we introduced a double-strand break (DSB) at a specific target site in a T. cruzi subtelomeric region cloned into an artificial chromosome (pTAC). This construct was used to transfect T. cruzi epimastigotes expressing the I-SceI meganuclease. Examination of the repaired sequences showed that DNA repair occurred only through homologous recombination (HR) with endogenous subtelomeric sequences. Our findings suggest that DSBs in subtelomeric repetitive sequences followed by HR between them may contribute to increased variability in T. cruzi multigene families.
Palabras clave: Artificial Chromosomes , Dna Repair , Double-Strand Break , Homologous Recombination , I-Scei Meganuclease , T. Cruzi , Telomere
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/40587
DOI: http://dx.doi.org/10.3389/fmicb.2016.02041
URL: https://www.frontiersin.org/articles/10.3389/fmicb.2016.02041/full
Colecciones
Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Chiurillo, Miguel A.; Barros, Roberto R. Moraes; Souza, Renata T.; Marini, Marjorie M.; Antonio, Cristiane R.; et al.; Subtelomeric I-SceI-mediated double-strand breaks are repaired by homologous recombination in Trypanosoma cruzi; Frontiers Research Foundation; Frontiers in Microbiology; 7; DEC; 12-2016; 1-8
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