Artículo
Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase
Zavala, Agustín; Kovacec, Verónica Ivana; Levín, Gustavo; Moglioni, Albertina Gladys
; Miranda, Maria Victoria
; García, Ernesto; Bonofiglio, Laura
; Mollerach, Marta Eugenia
Fecha de publicación:
01/2017
Editorial:
Taylor & Francis
Revista:
Journal of Enzyme Inhibition and Medicinal Chemistry
ISSN:
1475-6366
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The UDP-glucose pyrophosphorylase of Streptococcus pneumoniae (GalUSpn) is absolutely required for the biosynthesis of capsular polysaccharide, the sine qua non virulence factor of pneumococcus. Since the eukaryotic enzymes are completely unrelated to their prokaryotic counterparts, we propose that the GalU enzyme is a critical target to fight the pneumococcal disease. A recombinant GalUSpn was overexpressed and purified. An enzymatic assay that is rapid, sensitive and easy to perform was developed. This assay was appropriate for screening chemical libraries for searching GalU inhibitors. This work represents a fundamental step in the exploration of novel antipneumococcal drugs.
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Articulos(IQUIMEFA)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Zavala, Agustín; Kovacec, Verónica Ivana; Levín, Gustavo; Moglioni, Albertina Gladys; Miranda, Maria Victoria; et al.; Screening assay for inhibitors of a recombinant Streptococcus pneumoniae UDP-glucose pyrophosphorylase; Taylor & Francis; Journal of Enzyme Inhibition and Medicinal Chemistry; 32; 1; 1-2017; 203-207
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