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dc.contributor.author
Foscaldi, Sabrina Andrea
dc.contributor.author
D'antuono, Alejandra Lorena
dc.contributor.author
Noval, María Gabriela
dc.contributor.author
de Prat Gay, Gonzalo
dc.contributor.author
Scolaro, Luis Alberto
dc.contributor.author
Lopez, Nora Mabel
dc.date.available
2018-03-28T14:05:25Z
dc.date.issued
2017-07
dc.identifier.citation
Foscaldi, Sabrina Andrea; D'antuono, Alejandra Lorena; Noval, María Gabriela; de Prat Gay, Gonzalo; Scolaro, Luis Alberto; et al.; Regulation of Tacaribe mammarenavirus translation: Positive 5' and negative 3' elements and role of key cellular factors; American Society for Microbiology; Journal of Virology; 91; 14; 7-2017; 1-15; e00084-17
dc.identifier.issn
0022-538X
dc.identifier.uri
http://hdl.handle.net/11336/40344
dc.description.abstract
Mammarenaviruses are enveloped viruses with a bisegmented negativestranded RNA genome that encodes the nucleocapsid protein (NP), the envelope glycoprotein precursor (GPC), the RNA polymerase (L), and a RING matrix protein (Z). Viral proteins are synthesized from subgenomic mRNAs bearing a capped 5' untranslated region (UTR) and lacking 3' poly(A) tail. We analyzed the translation strategy of Tacaribe virus (TCRV), a prototype of the New World mammarenaviruses. A virus-like transcript that carries a reporter gene in place of the NP open reading frame and transcripts bearing modified 5' and/or 3' UTR were evaluated in a cellbased translation assay. We found that the presence of the cap structure at the 5' end dramatically increases translation efficiency and that the viral 5' UTR comprises stimulatory signals while the 3' UTR,specifically the presence of a terminal C+G-rich sequence and/or a stem-loop structure, down-modulates translation. Additionally, translation was profoundly reduced in eukaryotic initiation factor (eIF) 4G-inactivated cells, whereas depletion of intracellular levels of eIF4E had less impact on virus-like mRNA translation than on a cell-like transcript. Translation efficiency was independent of NP expression or TCRV infection. Our results indicate that TCRV mRNAs are translated using a cap-dependent mechanism, whose efficiency relies on the interplay between stimulatory signals in the 5' UTR and a negative modulatory element in the 3' UTR. The low dependence on eIF4E suggests that viral mRNAs may engage yet-unknown noncanonical host factors for a cap-dependent initiation mechanism.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arenavirus
dc.subject
Mrna
dc.subject
Translation
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Regulation of Tacaribe mammarenavirus translation: Positive 5' and negative 3' elements and role of key cellular factors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-27T14:49:57Z
dc.journal.volume
91
dc.journal.number
14
dc.journal.pagination
1-15; e00084-17
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Foscaldi, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
dc.description.fil
Fil: Noval, María Gabriela. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: de Prat Gay, Gonzalo. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lopez, Nora Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología ; Argentina
dc.journal.title
Journal of Virology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JVI.00084-17
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/content/91/14/e00084-17


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