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dc.contributor.author
Martinez, Carolina Soledad
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Piazza, Verónica Gabriela
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Gonzalez, Lorena
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Fang, Yimin
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Bartke, Andrzej
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Turyn, Daniel
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Miquet, Johanna Gabriela
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Sotelo, Ana Isabel
dc.date.available
2018-03-28T13:06:10Z
dc.date.issued
2016-03
dc.identifier.citation
Martinez, Carolina Soledad; Piazza, Verónica Gabriela; Gonzalez, Lorena; Fang, Yimin; Bartke, Andrzej; et al.; Mitogenic signaling pathways in the liver of growth hormone (GH)-overexpressing mice during the growth period; Taylor & Francis; Cell Cycle; 15; 5; 3-2016; 748-759
dc.identifier.issn
1538-4101
dc.identifier.uri
http://hdl.handle.net/11336/40334
dc.description.abstract
ABSTRACT: Growth hormone (GH) is a pleiotropic hormone that triggers STATs, ERK1/2 and Akt signaling, related to cell growth and proliferation. Transgenic mice overexpressing GH present increased body size, with a disproportionate liver enlargement due to hypertrophy and hyperplasia of the hepatocytes. We had described enhanced mitogenic signaling in liver of young adult transgenic mice. We now evaluate the activation of these signaling cascades during the growth period and relate them to the morphological alterations found. Signaling mediators, cell cycle regulators and transcription factors involved in cellular growth in the liver of GH-overexpressing growing mice were assessed by immunoblotting, RT-qPCR and immunohistochemistry. Hepatocyte enlargement can be seen as early as 2-weeks of age in GH-overexpressing animals, although it is more pronounced in young adults. Levels of cell cycle mediators PCNA and cyclin D1, and transcription factor c-Jun increase with age in transgenic mice with no changes in normal mice, whereas c-Myc levels are higher in 2-week-old transgenic animals and cyclin E levels decline with age for both genotypes. STAT3, Akt and GSK3 present higher activation in the adult transgenic mice than in the growing animals, while for c-Src and mTOR, phosphorylation in GH-overexpressing mice is higher than in control siblings at 4 and 9 weeks of age. No significant changes are observed for ERK1/2, neither by age or genotype. Thus, the majority of the mitogenic signaling pathways are gradually up-regulated in the liver of GH-transgenic mice, giving rise to the hepatic morphological changes these mice exhibit.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Akt/Mtor/Gsk3β
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C-Jun
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C-Myc
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Cell Cycle Regulators
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Erk1/2
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Gh-Overexpressing Mice
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Growth Hormone
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Hepatomegaly
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Liver
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Pcna
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Stat3
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Mitogenic signaling pathways in the liver of growth hormone (GH)-overexpressing mice during the growth period
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-15T14:06:14Z
dc.journal.volume
15
dc.journal.number
5
dc.journal.pagination
748-759
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Martinez, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Piazza, Verónica Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Gonzalez, Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Fang, Yimin. Southern Illinois University; Estados Unidos
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Fil: Bartke, Andrzej. Southern Illinois University; Estados Unidos
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Fil: Turyn, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Miquet, Johanna Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Sotelo, Ana Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.journal.title
Cell Cycle
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15384101.2016.1148844
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/15384101.2016.1148844