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dc.contributor.author
Moya, C. M.  
dc.contributor.author
Mendive, F. M.  
dc.contributor.author
Rivolta, Carina Marcela  
dc.contributor.author
Vassart, G.  
dc.contributor.author
Targovnik, Hector Manuel  
dc.date.available
2018-03-28T13:05:04Z  
dc.date.issued
2000-12  
dc.identifier.citation
Moya, C. M.; Mendive, F. M.; Rivolta, Carina Marcela; Vassart, G.; Targovnik, Hector Manuel; Genomic organization of the 5′ region of the human thyroglobulin gene; BioScientifica; European Journal of Endocrinology; 143; 6; 12-2000; 789-798  
dc.identifier.issn
0804-4643  
dc.identifier.uri
http://hdl.handle.net/11336/40328  
dc.description.abstract
Objective: The purpose of the present work is to establish the intron - exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5′ terminal half of the gene. Design: Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. Methods: A human genomic DNA library was screened by in situ hybridization. Southern blotting experiments were performed to characterize the phage inserts. Intron/exon junction sequences were determined by the Taq polymerase-based chain terminator method. Finally, the thyroglobulin gene was mapped using the Gene Bridge 4 radiation hybrid clone panel. Results: We isolated and characterized four λ phage clones that include nucleotides 3002 to 4816 of the thyroglobulin mRNA, encompassing exons 12 to 23 of the gene. The exon sizes range between 78 and 219 nucleotides. We found that the GT-AG splicing sequences rule was perfectly respected in all the introns. A total of 7302 intronic bases was analyzed. Hormogenic tyrosine 5 and 1291 are encoded by exons 2 and 18. Also, seven alternative spliced variants are associated with the 5′ region. Thyroglobulin gene maps to 5.5 centiRays from the AFMAO53XF1 marker, in chromosome 8. Conclusions: The present study shows that the first 4857 bases of thyroglobulin mRNA are divided into 23 exons and the four phages isolated include 32.6 kb genomic DNA, covering 1815 nucleotides of exonic sequence distributed in 12 exons, from exon 12 to 23.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
BioScientifica  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Genomic Organization  
dc.subject
Thyroglobulin Gene  
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5' Region  
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Exon/Intron  
dc.subject.classification
Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Genomic organization of the 5′ region of the human thyroglobulin gene  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-03-16T15:14:12Z  
dc.journal.volume
143  
dc.journal.number
6  
dc.journal.pagination
789-798  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Bristol  
dc.description.fil
Fil: Moya, C. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina  
dc.description.fil
Fil: Mendive, F. M.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina  
dc.description.fil
Fil: Rivolta, Carina Marcela. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Vassart, G.. Université Libre de Bruxelles; Bélgica  
dc.description.fil
Fil: Targovnik, Hector Manuel. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Université Libre de Bruxelles; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
European Journal of Endocrinology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1530/eje.0.1430789  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.eje-online.org/content/143/6/789