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dc.contributor.author
Krumschnabel, Gerhard
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dc.contributor.author
Schwarzbaum, Pablo Julio
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dc.contributor.author
Lisch, Johannes
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dc.contributor.author
Biasi, Christina
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dc.contributor.author
Wieser, Wolfgang
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dc.date.available
2018-03-28T13:04:39Z
dc.date.issued
2000-07
dc.identifier.citation
Krumschnabel, Gerhard; Schwarzbaum, Pablo Julio; Lisch, Johannes; Biasi, Christina; Wieser, Wolfgang; Oxygen-dependent energetics of anoxia-tolerant and anoxia-intolerant hepatocytes; Company of Biologists; Journal of Experimental Biology; 203; 7-2000; 951-959
dc.identifier.issn
0022-0949
dc.identifier.uri
http://hdl.handle.net/11336/40326
dc.description.abstract
The oxygen-dependence of cellular energetics was investigated in hepatocytes from goldfish Carassius auratus (anoxia-tolerant) and rainbow trout Oncorhynchus mykiss (anoxia-intolerant). In goldfish hepatocytes, an approximately 50 % reduction in the rate of oxygen consumption was observed in response to both acute and prolonged hypoxia, the latter treatment shifting the threshold for this reduction to a higher oxygen level. A concomitant increase in the rate of lactate production did not compensate for the decreased aerobic ATP supply, resulting in an overall metabolic depression of 26 % during acute hypoxia and of 42 % during prolonged hypoxia. Trout hepatocytes showed a similar suppression of cellular respiration after prolonged hypoxia but were unresponsive to acute hypoxia. Similarly, the rate of lactate production was unaltered during acute hypoxia but was increased during prolonged hypoxia, metabolic depression amounting to 7 % during acute hypoxia and 30 % during prolonged hypoxia. In both species, the affinity of hepatocytes for oxygen decreased during hypoxia, but this alteration was not sufficient in absolute terms to account for the observed decrease in aerobic ATP supply. Protein synthesis was suppressed in both cell types under hypoxia, whereas Na(+)/K(+)-ATPase activity decreased in trout but not in goldfish hepatocytes, emphasising the importance of membrane function in these cells during conditions of limited energy supply.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oxidative Metabolism
dc.subject
Ion Regulation
dc.subject.classification
Biología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Oxygen-dependent energetics of anoxia-tolerant and anoxia-intolerant hepatocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-16T15:14:10Z
dc.identifier.eissn
1477-9145
dc.journal.number
203
dc.journal.pagination
951-959
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Krumschnabel, Gerhard. Universidad de Innsbruck; Austria
dc.description.fil
Fil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Lisch, Johannes. Universidad de Innsbruck; Austria
dc.description.fil
Fil: Biasi, Christina. Universidad de Innsbruck; Austria
dc.description.fil
Fil: Wieser, Wolfgang. Universidad de Innsbruck; Austria
dc.journal.title
Journal of Experimental Biology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jeb.biologists.org/content/203/5/951
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