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Artículo

Kinetics of human sperm acrosomal exocytosis

Sosa, Claudia MarcelaIcon ; Pavarotti, Martin AlejandroIcon ; Zanetti, Maria NataliaIcon ; Zoppino, Felipe Carlos MartinIcon ; de Blas, Gerardo AndrésIcon ; Mayorga, Luis SegundoIcon
Fecha de publicación: 01/09/2014
Editorial: Oxford University Press
Revista: Molecular Human Reproduction
ISSN: 1360-9947
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

The acrosome reaction is a unique event in the lifespan of sperm characterized by the exocytosis of the acrosomal content and the release of hybrid vesicles formed by patches of the outer acrosomal membrane and the plasma membrane. This unique regulated exocytosis is mediated by essentially the same membrane fusion machinery present in neuroendocrine cells. However, whereas secretion in neuroendocrine cells occurs in less than a second, the acrosome reaction is normally assessed after several minutes of incubation with inducers. In this report, we measured the kinetics of human sperm exocytosis triggered by two stimuli (calcium ionophore and progesterone) by using electron microscopy and three different approaches based on the incorporation of fluorescent Pisum sativum agglutinin into the acrosome upon opening of fusion pores connecting the extracellular medium with the acrosomal lumen. The results with the different methods are consistent with a slow kinetics (t1/2 = 14 min). We also manipulated the system to measure different steps of the process. We observed that cytosolic calcium increased with a relatively fast kinetics (t1/2 = 0.1 min). In contrast, the swelling of the acrosomal granule that precedes exocytosiswas a slow process (t1/2 = 13 min). Whenswelling wascompleted, the fusion pore opening wasfast (t1/2 = 0.2 min). The results indicate that acrosomal swelling is the slowest step and it determines the kinetics of the acrosome reaction. After the swelling is completed, the efflux of calcium from intracellular stores triggers fusion pores opening and the release of hybrid vesicles in seconds.
Palabras clave: Acrosomal Swelling , Acrosome Reaction , Fusion Pore , Regulated Exocytosis , Secretion Kinetics
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/40296
URL: https://academic.oup.com/molehr/article/21/3/244/1141025
DOI: https://doi.org/10.1093/molehr/gau110
Colecciones
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Sosa, Claudia Marcela; Pavarotti, Martin Alejandro; Zanetti, Maria Natalia; Zoppino, Felipe Carlos Martin; de Blas, Gerardo Andrés; et al.; Kinetics of human sperm acrosomal exocytosis; Oxford University Press; Molecular Human Reproduction; 21; 3; 1-9-2014; 244-254
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