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dc.contributor.author
Furtado, Ana Rita
dc.contributor.author
Essid, Miriam
dc.contributor.author
Perrinet, Stéphenie
dc.contributor.author
Balaña, Maria Eugenia
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dc.contributor.author
Yoder, Nicholas
dc.contributor.author
Dehoux, Pierre
dc.contributor.author
Subtil, Agathe
dc.date.available
2016-02-04T20:28:27Z
dc.date.issued
2013-08
dc.identifier.citation
Furtado, Ana Rita; Essid, Miriam; Perrinet, Stéphenie; Balaña, Maria Eugenia; Yoder, Nicholas; et al.; The chlamydial OTU domain-containing protein ChlaOTU is an early type III secretion effector targeting ubiquitin and NDP52; Wiley; Cellular Microbiology (print); 15; 12; 8-2013; 2064–2079
dc.identifier.issn
1462-5814
dc.identifier.uri
http://hdl.handle.net/11336/4027
dc.description.abstract
Chlamydia are obligate intracellular pathogens. Upon contact with the host, they use type III secretion to deliver proteins into the cell, thereby triggering actin-dependent entry and establishing the infection. We observed that Chlamydia caviae elicited a local and transient accumulation of ubiquitinated proteins at the entry sites, which disappeared within 20 min. We investigated the mechanism for the rapid clearance of ubiquitin. We showed that the OTU-like domain containing protein CCA00261, predicted to have deubiquitinase activity, was detected in infectious particles and was a type III secretion effector. This protein is present in several Chlamydia strains, including the human pathogen Chlamydia pneumoniae, and we further designate it as ChlaOTU. We demonstrated that ChlaOTU bound ubiquitin and NDP52, and we mapped these interactions to distinct domains. NDP52 was recruited to Chlamydia entry sites and was dispensable for infection and for bacterial growth. ChlaOTU functioned as a deubiquitinase in vitro. Heterologousexpression of ChlaOTU reduced ubiquitin accumulation at the entry sites, while a catalytic mutant of the deubiquitinase activity had the opposite effect. Altogether, we have identified a novel secreted protein of chlamydiae. ChlaOTU targets both ubiquitin and NDP52 and likely participates in the clearance of ubiquitin at the invasion sites.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Chlamydia
dc.subject
Ubiquitin
dc.subject
Invasion Sites
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The chlamydial OTU domain-containing protein ChlaOTU is an early type III secretion effector targeting ubiquitin and NDP52
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
15
dc.journal.number
12
dc.journal.pagination
2064–2079
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Furtado, Ana Rita. Institut Pasteur. Unité de Biologie des Interactions Cellulaires; Francia
dc.description.fil
Fil: Essid, Miriam. Institut Pasteur. Unité de Biologie des Interactions Cellulaires; Francia
dc.description.fil
Fil: Perrinet, Stéphenie. Institut Pasteur. Unité de Biologie des Interactions Cellulaires; Francia
dc.description.fil
Fil: Balaña, Maria Eugenia. Institut Pasteur. Unité de Biologie des Interactions Cellulaires; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.description.fil
Fil: Yoder, Nicholas. Nine Cambridge Center. Whitehead Institute for Biomedical Research; Estados Unidos
dc.description.fil
Fil: Dehoux, Pierre. Institut Pasteur. Plateforme Intégration et Analyse Génomique; Francia
dc.description.fil
Fil: Subtil, Agathe. Institut Pasteur. Unité de Biologie des Interactions Cellulaires; Francia
dc.journal.title
Cellular Microbiology (print)
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/cmi.12171/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1111/cmi.12171
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/1462-5814
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