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Artículo

Coronatine inhibits stomatal closure through guard cell-specific inhibition of NADPH oxidase-dependent ROS production

Toum, LailaIcon ; Torres, Pablo SebastianIcon ; Gallego, Susana MabelIcon ; Benavides, Maria PatriciaIcon ; Vojnov, Adrián AlbertoIcon ; Gudesblat, Gustavo EduardoIcon
Fecha de publicación: 12/2016
Editorial: Frontiers Research Foundation
Revista: Frontiers in Plant Science
ISSN: 1664-462X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Microbes trigger stomatal closure through microbe-associated molecular patterns (MAMPs). The bacterial pathogen Pseudomonas syringae pv. tomato (Pst) synthesizes the polyketide toxin coronatine, which inhibits stomatal closure by MAMPs and by the hormone abscisic acid (ABA). The mechanism by which coronatine, a jasmonic acid-isoleucine analog, achieves this effect is not completely clear. Reactive oxygen species (ROS) are essential second messengers in stomatal immunity, therefore we investigated the possible effect of coronatine on their production. We found that coronatine inhibits NADPH oxidase-dependent ROS production induced by ABA, and by the flagellin-derived peptide flg22. This toxin also inhibited NADPH oxidase-dependent stomatal closure induced by darkness, however, it failed to prevent stomatal closure by exogenously applied H2 O2 or by salicylic acid, which induces ROS production through peroxidases. Contrary to what was observed on stomata, coronatine did not affect the oxidative burst induced by flg22 in leaf disks. Additionally, we observed that in NADPH oxidase mutants atrbohd and atrbohd/f, as well as in guard cell ABA responsive but flg22 insensitive mutants mpk3, mpk6, npr1-3, and lecrk-VI.2-1, the inhibition of ABA stomatal responses by both coronatine and the NADPH oxidase inhibitor diphenylene iodonium was markedly reduced. Interestingly, coronatine still impaired ABA-induced ROS synthesis in mpk3, mpk6, npr1-3, and lecrk-VI.2-1, suggesting a possible feedback regulation of ROS on other guard cell ABA signaling elements in these mutants. Altogether our results show that inhibition of NADPH oxidase-dependent ROS synthesis in guard cells plays an important role during endophytic colonization by Pst through stomata.
Palabras clave: Abscisic Acid , Arabidopsis Thaliana , Coronatine , Nadph Oxidase , Reactive Oxygen Species , Stomata
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/40178
DOI: http://dx.doi.org/10.3389/fpls.2016.01851
URL: https://www.frontiersin.org/articles/10.3389/fpls.2016.01851/full
Colecciones
Articulos(IBBEA)
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos(ICT - MILSTEIN)
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Toum, Laila; Torres, Pablo Sebastian; Gallego, Susana Mabel; Benavides, Maria Patricia; Vojnov, Adrián Alberto; et al.; Coronatine inhibits stomatal closure through guard cell-specific inhibition of NADPH oxidase-dependent ROS production; Frontiers Research Foundation; Frontiers in Plant Science; 7; DECEMBER2016; 12-2016; 1-12; 1851
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