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dc.contributor.author
Deng, Lei
dc.contributor.author
Ibañez, Lorena Itatí
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dc.contributor.author
Van Den Bossche, Veronique
dc.contributor.author
Roose, Kenny
dc.contributor.author
Youssef, Sameh A.
dc.contributor.author
De Bruin, Alain
dc.contributor.author
Fiers, Walter
dc.contributor.author
Saelens, Xavier
dc.date.available
2018-03-27T18:06:45Z
dc.date.issued
2015-05
dc.identifier.citation
Deng, Lei; Ibañez, Lorena Itatí; Van Den Bossche, Veronique; Roose, Kenny; Youssef, Sameh A.; et al.; Protection against influenza a virus challenge with M2e-displaying filamentous Escherichia coli phages; Public Library of Science; Plos One; 10; 5; 5-2015; 1-21; e0126650
dc.identifier.uri
http://hdl.handle.net/11336/40175
dc.description.abstract
Human influenza viruses are responsible for annual epidemics and occasional pandemics that cause severe illness and mortality in all age groups worldwide. Matrix protein 2 (M2) of influenza A virus is a tetrameric type III membrane protein that functions as a proton-selective channel. The extracellular domain of M2 (M2e) is conserved in human and avian influenza A viruses and is being pursued as a component for a universal influenza A vaccine. To develop a M2e vaccine that is economical and easy to purify, we genetically fused M2e amino acids 2-16 to the N-terminus of pVIII, the major coat protein of filamentous bacteriophage f88. We show that the resulting recombinant f88-M2e2-16 phages are replication competent and display the introduced part of M2e on the phage surface. Immunization of mice with purified f88-M2e2-16 phages in the presence of incomplete Freund's adjuvant, induced robust M2e-specific serum IgG and protected BALB/c mice against challenge with human and avian influenza A viruses. Thus, replication competent filamentous bacteriophages can be used as efficient and economical carriers to display conserved B cell epitopes of influenza A.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
M2e
dc.subject
Influenza
dc.subject
Phages
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Vaccines
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Protection against influenza a virus challenge with M2e-displaying filamentous Escherichia coli phages
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-27T14:48:15Z
dc.identifier.eissn
1932-6203
dc.journal.volume
10
dc.journal.number
5
dc.journal.pagination
1-21; e0126650
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Deng, Lei. University of Ghent; Bélgica
dc.description.fil
Fil: Ibañez, Lorena Itatí. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Van Den Bossche, Veronique. University of Ghent; Bélgica
dc.description.fil
Fil: Roose, Kenny. University of Ghent; Bélgica
dc.description.fil
Fil: Youssef, Sameh A.. Utrecht University; Países Bajos
dc.description.fil
Fil: De Bruin, Alain. Utrecht University; Países Bajos
dc.description.fil
Fil: Fiers, Walter. University of Ghent; Bélgica
dc.description.fil
Fil: Saelens, Xavier. University of Ghent; Bélgica
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0126650
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0126650
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