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dc.contributor.author
García, Alejandro
dc.contributor.author
Llorente, Constanza Gabriela
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dc.contributor.author
Cuello, María T.
dc.contributor.author
dos Santos, Mariana
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Elsner, Boris
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dc.contributor.author
Avagnina, Alejandra
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dc.contributor.author
Denninghoff, Valeria Cecilia
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dc.date.available
2018-03-27T17:57:38Z
dc.date.issued
2017-02
dc.identifier.citation
García, Alejandro; Llorente, Constanza Gabriela; Cuello, María T.; dos Santos, Mariana; Elsner, Boris; et al.; Performance Assessment of Epidermal Growth Factor Receptor Gene Sequencing According to Sample Size in Daily Practice Conditions; Lippincott Williams; Applied Immunohistochemistry & Molecular Morphology; 2-2017
dc.identifier.issn
1062-3345
dc.identifier.uri
http://hdl.handle.net/11336/40173
dc.description.abstract
Lung carcinoma is the main cause of cancer death worldwide. Adenocarcinoma molecular biomarkers have been discovered, and targeted therapies have been developed with encouraging results. The epidermal growth factor receptor gene is one of these biomarkers. Exons 18 to 21 should be studied in patients with advanced adenocarcinoma, who are candidates for treatment with tyrosine kinase inhibitors. The objective was to compare the performance of the determination in large and small samples in daily practice conditions, trying to adjust to published consensus guidelines. A retrospective observational study of 141 cases was carried out, with exons 19 and 21 sequencing. Sample size (small vs. large), including number of satisfactory polymerase chain reaction (PCR), sequencing, deletions, and mutations, were evaluated. In small biopsies, sample type, fragment number, and percentage of tumor per sample were analyzed. The results shown 114/141 (80.8) cases that met selection criteria; 60/114 (53%) were large (surgical) and 54/114 (47%) were small samples (19/54 endoscopic, 17/54 fine needle aspiration clots, 4/54 lymph nodes, 14/54 core and other). All large samples were satisfactory PCR, 56/60 (93%) satisfactory sequencing, and 12/56 (21%) had deletions in exon 19. Small samples were satisfactory PCRs in 50/54 (93%) cases, and satisfactory sequencing in 35/50 (65%), 8/35 (23%) showed alterations in exon 19, and 1/35 (3%) in exon 21. In conclusion, the proportion of samples unfit for the study of the epidermal growth factor receptor gene mutational status increased from 7% in large samples to 35% in small ones. Nineteen small samples were inconclusive, with cell blocks predominating, 10/19 (53%).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Lippincott Williams
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Egfr
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Small Samples
dc.subject
Adenocarcinoma
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Lung
dc.subject.classification
Medicina Critica y de Emergencia
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Medicina Clínica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Performance Assessment of Epidermal Growth Factor Receptor Gene Sequencing According to Sample Size in Daily Practice Conditions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-27T14:49:48Z
dc.identifier.eissn
1533-4058
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Filadelfia
dc.description.fil
Fil: García, Alejandro. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
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Fil: Llorente, Constanza Gabriela. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
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Fil: Cuello, María T.. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
dc.description.fil
Fil: dos Santos, Mariana. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
dc.description.fil
Fil: Elsner, Boris. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
dc.description.fil
Fil: Avagnina, Alejandra. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
dc.description.fil
Fil: Denninghoff, Valeria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas ; Argentina
dc.journal.title
Applied Immunohistochemistry & Molecular Morphology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1097/PAI.0000000000000455
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/crossref?an=00129039-900000000-98970
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/appliedimmunohist/Abstract/publishahead/Performance_Assessment_of_Epidermal_Growth_Factor.98970.aspx
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