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dc.contributor.author
Giannakopoulou, Artemis
dc.contributor.author
Steele, John F. C.
dc.contributor.author
Segretin, Maria Eugenia
dc.contributor.author
Bozkurt, Tolga
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Zhou, Ji
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Robatzek, Silke
dc.contributor.author
Banfield, Mark J
dc.contributor.author
Pais, Marina
dc.contributor.author
Kamoun, Sophien
dc.date.available
2016-02-03T20:15:04Z
dc.date.issued
2015-09
dc.identifier.citation
Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; et al.; Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 28; 12; 9-2015; 1316-1329
dc.identifier.issn
0894-0282
dc.identifier.uri
http://hdl.handle.net/11336/4001
dc.description.abstract
Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nb-Lrr
dc.subject
R3a
dc.subject
Phytophthora Infestans
dc.subject
I2
dc.subject.classification
Ciencias de las Plantas, Botánica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
28
dc.journal.number
12
dc.journal.pagination
1316-1329
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Saint Paul
dc.description.fil
Fil: Giannakopoulou, Artemis. The Sainsbury Laboratory; Reino Unido
dc.description.fil
Fil: Steele, John F. C.. John Innes Institute; Reino Unido
dc.description.fil
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Bozkurt, Tolga. The Sainsbury Laboratory; Reino Unido. Imperial College London; Reino Unido
dc.description.fil
Fil: Zhou, Ji. The Sainsbury Laboratory; Reino Unido. The Genome Analysis Centre; Reino Unido
dc.description.fil
Fil: Robatzek, Silke. The Sainsbury Laboratory; Reino Unido
dc.description.fil
Fil: Banfield, Mark J. John Innes Institute; Reino Unido
dc.description.fil
Fil: Pais, Marina. The Sainsbury Laboratory; Reino Unido
dc.description.fil
Fil: Kamoun, Sophien. The Sainsbury Laboratory; Reino Unido
dc.journal.title
Molecular Plant-Microbe Interactions
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-07-15-0147-R
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/MPMI-07-15-0147-R
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/0894-0282


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