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dc.contributor.author Giannakopoulou, Artemis
dc.contributor.author Steele, John F. C.
dc.contributor.author Segretin, Maria Eugenia
dc.contributor.author Bozkurt, Tolga
dc.contributor.author Zhou, Ji
dc.contributor.author Robatzek, Silke
dc.contributor.author Banfield, Mark J
dc.contributor.author Pais, Marina
dc.contributor.author Kamoun, Sophien
dc.date.available 2016-02-03T20:15:04Z
dc.date.issued 2015-09
dc.identifier.citation Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; et al.; Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 28; 12; 9-2015; 1316-1329
dc.identifier.issn 0894-0282
dc.identifier.uri http://hdl.handle.net/11336/4001
dc.description.abstract Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Phytopathological Society
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject NB-LRR
dc.subject R3a
dc.subject PHYTOPHTHORA INFESTANS
dc.subject I2
dc.subject.classification Ciencias de las Plantas, Botánica
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.journal.volume 28
dc.journal.number 12
dc.journal.pagination 1316-1329
dc.journal.pais Estados Unidos
dc.journal.ciudad Saint Paul
dc.description.fil Fil: Giannakopoulou, Artemis. The Sainsbury Laboratory; Reino Unido
dc.description.fil Fil: Steele, John F. C.. John Innes Institute; Reino Unido
dc.description.fil Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil Fil: Bozkurt, Tolga. The Sainsbury Laboratory; Reino Unido. Imperial College London; Reino Unido
dc.description.fil Fil: Zhou, Ji. The Sainsbury Laboratory; Reino Unido. The Genome Analysis Centre; Reino Unido
dc.description.fil Fil: Robatzek, Silke. The Sainsbury Laboratory; Reino Unido
dc.description.fil Fil: Banfield, Mark J. John Innes Institute; Reino Unido
dc.description.fil Fil: Pais, Marina. The Sainsbury Laboratory; Reino Unido
dc.description.fil Fil: Kamoun, Sophien. The Sainsbury Laboratory; Reino Unido
dc.journal.title Molecular Plant-Microbe Interactions
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-07-15-0147-R
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/MPMI-07-15-0147-R
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/issn/0894-0282


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)