Artículo
Mapping Antigenic Motifs in the Trypomastigote Small Surface Antigen from Trypanosoma cruzi
Balouz, Virginia
; Camara, María de los Milagros
; Canepa, Gaspar Exequiel
; Carmona, Santiago Javier
; Volcovich, Romina; González, Nicolás; Altcheh, Jaime Marcelo
; Agüero, Fernan Gonzalo
; Buscaglia, Carlos Andres
Fecha de publicación:
01/2015
Editorial:
American Society for Microbiology
Revista:
Clinical and Vaccine Immunology
ISSN:
1556-6811
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The trypomastigote small surface antigen (TSSA) is a mucin-like molecule from Trypanosoma cruzi, the etiological agent of Chagas disease, which displays amino acid polymorphisms in parasite isolates. TSSA expression is restricted to the surface of infective cell-derived trypomastigotes, where it functions as an adhesin and engages surface receptors on the host cell as a prerequisite for parasite internalization. Previous results have established TSSA-CL, the isoform encoded by the CL Brener clone, as an appealing candidate for use in serology-based diagnostics for Chagas disease. Here, we used a combination of peptide- and recombinant protein-based tools to map the antigenic structure of TSSA-CL at maximal resolution. Our results indicate the presence of different partially overlapping B-cell epitopes clustering in the central portion of TSSA-CL, which contains most of the polymorphisms found in parasite isolates. Based on these results, we assessed the serodiagnostic performance of a 21-amino-acid-long peptide that spans TSSA-CL major antigenic determinants, which was similar to the performance of the previously validated glutathione S-transferase (GST)-TSSA-CL fusion molecule. Furthermore, the tools developed for the antigenic characterization of the TSSA antigen were also used to explore other potential diagnostic applications of the anti-TSSA humoral response in Chagasic patients. Overall, our present results provide additional insights into the antigenic structure of TSSA-CL and support this molecule as an excellent target for molecular intervention in Chagas disease.
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Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Balouz, Virginia; Camara, María de los Milagros; Canepa, Gaspar Exequiel; Carmona, Santiago Javier; Volcovich, Romina; et al.; Mapping Antigenic Motifs in the Trypomastigote Small Surface Antigen from Trypanosoma cruzi; American Society for Microbiology; Clinical and Vaccine Immunology; 22; 3; 1-2015; 304-312
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