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dc.contributor.author
Soto Vargas, Verónica Carolina  
dc.contributor.author
Jofre, Viviana Patricia  
dc.contributor.author
Galmarini, Claudio Romulo  
dc.contributor.author
Silva, María Fernanda  
dc.date.available
2018-03-26T17:32:41Z  
dc.date.issued
2016-07  
dc.identifier.citation
Soto Vargas, Verónica Carolina; Jofre, Viviana Patricia; Galmarini, Claudio Romulo; Silva, María Fernanda; Determination of alkaloids in onion nectar by micellar electrokinetic chromatography; Wiley VCH Verlag; Electrophoresis; 37; 13; 7-2016; 1741-1966  
dc.identifier.issn
0173-0835  
dc.identifier.uri
http://hdl.handle.net/11336/39922  
dc.description.abstract
Nectar is the most important floral reward offered by plants to insects. Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. Considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar by MEKC-UV is herein reported. The extraction of alkaloid compounds in nectar was performed by SPE using a homemade miniaturized column (C18). Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire optimum conditions. Under the proposed conditions, the analytes can be separated within 15 min in a 50 cm effective length capillary (75 μm id) at a separation voltage of 20 kV in 20 mmol/L sodium tretraborate, 100 mmol/L SDS. The amount of sample requirement was reduced up to 2000 times, when compared to traditional methods, reaching limits of detection as low as 0.0153 ng/L. For the first time, this study demonstrates that there are marked qualitative and quantitative differences in nectar alkaloids between open pollinated and male sterile lines (MSLs) and also within MSLs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley VCH Verlag  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Allium Cepa L  
dc.subject
Male Sterility  
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Nectar Alkaloids  
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Seed Yield  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Determination of alkaloids in onion nectar by micellar electrokinetic chromatography  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-03-23T18:18:23Z  
dc.journal.volume
37  
dc.journal.number
13  
dc.journal.pagination
1741-1966  
dc.journal.pais
Alemania  
dc.journal.ciudad
Weinheim  
dc.description.fil
Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.description.fil
Fil: Jofre, Viviana Patricia. Instituto Nacional de Tecnología Agropecuaria; Argentina  
dc.description.fil
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina  
dc.description.fil
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina  
dc.journal.title
Electrophoresis  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/elps.201600060  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201600060