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dc.contributor.author
Peichoto, María Elisa
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dc.contributor.author
Santoro, Marcelo Larami
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dc.date.available
2018-03-20T20:48:34Z
dc.date.issued
2016-09
dc.identifier.citation
Peichoto, María Elisa; Santoro, Marcelo Larami; Patagonfibrase modifies protein expression of tissue factor and protein disulfide isomerase in rat skin; Pergamon-Elsevier Science Ltd; Toxicon; 119; 9-2016; 330-335
dc.identifier.issn
0041-0101
dc.identifier.uri
http://hdl.handle.net/11336/39437
dc.description.abstract
Patagonfibrase is a hemorrhagic metalloproteinase isolated from the venom of the South American rear-fanged snake Philodryas patagoniensis, and is an important contributor to local lesions inflicted by this species. The tissue factor (TF)-factor VIIa complex, besides triggering the coagulation cascade, has been demonstrated to be involved in inflammatory events. Our aim was to determine whether patagonfibrase affects the expression of TF and protein disulfide isomerase (PDI), an enzyme that controls TF biological activity, at the site of patagonfibrase injection, and thus if they may play a role in hemostatic and inflammatory events induced by snake venoms. Patagonfibrase (60 μg/kg) was administered s.c. to rats, and after 3 h blood was collected to evaluate hemostasis parameters, and skin fragments close to the site of injection were taken to assess TF and PDI expression. Patagonfibrase did not alter blood cell counts, plasma fibrinogen levels, or levels of TF activity in plasma. However, by semiquantitative Western blotting, patagonfibrase increased TF expression by 2-fold, and decreased PDI expression by 3-fold in skin samples. In agreement, by immunohistochemical analyses, prominent TF expression was observed in the subcutaneous tissue. Thus, patagonfibrase affects the local expression of TF and PDI without inducing any systemic hemostatic disturbance, although that they may be involved in the local inflammatory events induced by hemorrhagic metalloproteinases. Once antivenom therapy is not totally effective to treat the local injury induced by snake venoms, modulation of the activity and expression of TF and/or PDI might become a strategy for treating snake envenomation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bothrops
dc.subject
Colubridae
dc.subject
Hemostasis
dc.subject
Inflammation
dc.subject
P4hb
dc.subject
Platelets
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Patagonfibrase modifies protein expression of tissue factor and protein disulfide isomerase in rat skin
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-20T13:12:14Z
dc.identifier.eissn
1879-3150
dc.journal.volume
119
dc.journal.pagination
330-335
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
dc.journal.title
Toxicon
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.toxicon.2016.07.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010116302008
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