Mostrar el registro sencillo del ítem
dc.contributor.author
Barata Vallejo, Sebastian
dc.contributor.author
Ferreri, Carla
dc.contributor.author
Zhang, Tao
dc.contributor.author
Permentier, Hjalmar
dc.contributor.author
Bischoff, Rainer
dc.contributor.author
Bobrowski, Krzysztof
dc.contributor.author
Chatgilialoglu, Chryssostomos
dc.date.available
2018-03-20T18:04:21Z
dc.date.issued
2016-11
dc.identifier.citation
Barata Vallejo, Sebastian; Ferreri, Carla; Zhang, Tao; Permentier, Hjalmar; Bischoff, Rainer; et al.; Radiation chemical studies of Gly-Met-Gly in aqueous solution; Taylor & Francis Ltd; Free Radical Research; 50; 11-2016; S24-S39
dc.identifier.issn
1071-5762
dc.identifier.uri
http://hdl.handle.net/11336/39379
dc.description.abstract
Important biological consequences are related to the reaction of HO• radicals with methionine (Met). Several fundamental aspects remain to be defined when Met is an amino acid residue incorporated in the interior of peptides and proteins. The present study focuses on Gly-Met-Gly, the simplest peptide where Met is not a terminal residue. The reactions of HO• with Gly-Met-Gly and its N-acetyl derivative were studied by pulse radiolysis technique. The transient absorption spectra were resolved into contributions from specific components of radical intermediates. Moreover, a detailed product analysis is provided for the first time for Met-containing peptides in radiolytic studies to support the mechanistic proposal. By parallel radiolytical and electrochemical reactions and consequent product identification, the formation of sulfoxide attributed to the direct HO• radical attack on the sulfide functionality of the Met residue could be excluded, with the in situ generated hydrogen peroxide responsible for this oxidation. LC–MS and high resolution MS/MS were powerful analytical tools to envisage the structures of five products, thus allowing to complete the mechanistic picture of the overall Met-containing peptide reactivity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ec-Ms
dc.subject
Electrochemistry
dc.subject
Free Radicals
dc.subject
Ionizing Radiation
dc.subject
Methionine
dc.subject
Peptide
dc.subject
Reaction Mechanism
dc.subject.classification
Otras Ciencias Químicas
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Radiation chemical studies of Gly-Met-Gly in aqueous solution
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-20T14:32:56Z
dc.journal.volume
50
dc.journal.pagination
S24-S39
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
dc.description.fil
Fil: Zhang, Tao. University of Groningen; Países Bajos
dc.description.fil
Fil: Permentier, Hjalmar. University of Groningen; Países Bajos
dc.description.fil
Fil: Bischoff, Rainer. University of Groningen; Países Bajos
dc.description.fil
Fil: Bobrowski, Krzysztof. University of Notre Dame; Estados Unidos. Institute of Nuclear Chemistry and Technology; Polonia
dc.description.fil
Fil: Chatgilialoglu, Chryssostomos. Consiglio Nazionale delle Ricerche; Italia. Institute of Nanoscience and Nanotechnology; Grecia
dc.journal.title
Free Radical Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/10715762.2016.1231402
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/10715762.2016.1231402
Archivos asociados