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dc.contributor.author
Waghmare, Ashish G.
dc.contributor.author
Salve, Manoj K.
dc.contributor.author
Leblanc, Jean Guy Joseph
dc.contributor.author
Arya, Shalini S.
dc.date.available
2018-03-20T14:35:00Z
dc.date.issued
2016-03-26
dc.identifier.citation
Waghmare, Ashish G.; Salve, Manoj K.; Leblanc, Jean Guy Joseph; Arya, Shalini S.; Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa; Springer Open; Bioresources and Bioprocessing; 3; 1; 26-3-2016; 1-11
dc.identifier.issn
2197-4365
dc.identifier.uri
http://hdl.handle.net/11336/39336
dc.description.abstract
Background: Many methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very time-consuming. Three-phase partitioning (TPP) is a separation technique that has gained a lot of interest due to its rapid, simple and scalable use for concentration, isolation and decontamination of proteins from crude samples with high recovery yields. In the present work, the effect of various parameters of TPP was evaluated to optimize the concentration of proteins from Chlorella pyrenoidosa (CP), is green algae that increasingly being used as food supplements because of its positive impacts on human health. Results: Chlorella pyrenoidosa was cultivated in a closed system under controlled conditions. After reaching maximum growth, the microalgae was harvested, dried and powdered. Afterwards, TPP of CP cell lysate was done to concentrate protein content. To maximize protein concentration, various parameters were optimized such as solvent (t-butanol), ammonium sulphate concentration (40 % w/v), solid load (0.75 g/20 mL), pH (6), incubation time (20 min), slurry to butanol ratio (1:1.5) and enzymatic treatment (combination of Stargen™ and Carezyme™). Also, total starch, cellulose and carbohydrate content before and after the enzymatic treatment were determined to comprehend the impact of enzymatic treatment on protein concentration. Using these optimized parameters, 78.1 % w/w protein concentration was obtained in middle protein concentrate phase. This protein concentrate was characterizedfor proximate composition, colour analysis, water holding capacity, oil-holding capacity, foaming capacity, foam stability, amino acid composition, protein quality and thermal properties. Conclusion: Various process parameters of TPP influence the protein concentration of middle protein concentrate phase. Enzymatically treated biomass also enhanced protein concentration in middle protein concentrate phase. Characterization of protein concentrate revealed the presence high-quality protein. Therefore, it is possible to implement TPP at an industrial scale for protein concentration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Open
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Microalgae
dc.subject
Three-Phase Partitioning
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Protein Concentrate
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Enzymatic Treatment
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Protein Quality
dc.title
Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-17T13:47:31Z
dc.journal.volume
3
dc.journal.number
1
dc.journal.pagination
1-11
dc.journal.pais
Alemania
dc.journal.ciudad
Heideberg
dc.description.fil
Fil: Waghmare, Ashish G.. Institute of Chemical Technology. Food Engineering and Technology Department; India
dc.description.fil
Fil: Salve, Manoj K.. Institute of Chemical Technology. Food Engineering and Technology Department; India
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Arya, Shalini S.. Institute of Chemical Technology. Food Engineering and Technology Department; India
dc.journal.title
Bioresources and Bioprocessing
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s40643-016-0094-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bioresourcesbioprocessing.springeropen.com/articles/10.1186/s40643-016-0094-8
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