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dc.contributor.author
Cabrillana, María Eugenia
dc.contributor.author
Monclus, Maria de Los Angeles
dc.contributor.author
Saez Lancellotti, Tania Emilce Estefania
dc.contributor.author
Boarelli, Paola Vanina
dc.contributor.author
Vincenti, Amanda Edith
dc.contributor.author
Fornes, Miguel Matías
dc.contributor.author
Sanabria, Eduardo Alfredo
dc.contributor.author
Fornes, Miguel Walter
dc.date.available
2018-03-19T17:33:59Z
dc.date.issued
2016-07-01
dc.identifier.citation
Cabrillana, María Eugenia; Monclus, Maria de Los Angeles; Saez Lancellotti, Tania Emilce Estefania; Boarelli, Paola Vanina; Vincenti, Amanda Edith; et al.; Thiols of flagellar proteins are essential for progressive motility in human spermatozoa; Csiro Publishing; Reproduction Fertility and Development; 29; 7; 1-7-2016; 1435-1446
dc.identifier.issn
1031-3613
dc.identifier.uri
http://hdl.handle.net/11336/39239
dc.description.abstract
Male infertility is a disorder of the reproductive system defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse. The presence of low-motile or immotile spermatozoa is one of many causes of infertility; however, this observation provides little or no information regarding the pathogenesis of the malfunction. Good sperm motility depends on correct assembly of the sperm tail in the testis and efficient maturation during epididymal transit. Thiols of flagellar proteins, such as outer dense fibre protein 1 (ODF1), are oxidised to form disulfides during epididymal transit and the spermatozoa become motile. This study was designed to determine how oxidative changes in protein thiol status affect progressive motility in human spermatozoa. Monobromobimane (mBBr) was used as a specific thiol marker and disruptor of sperm progressive motility. When mBBr was blocked by dithiothreitol it did not promote motility changes. The analysis of mBBr-treated spermatozoa revealed a reduction of progressive motility and an increased number of spermatozoa with non-progressive motility without affecting ATP production. Laser confocal microscopy and western blot analysis showed that one of the mBBr-positive proteins reacted with an antibody to ODF1. Monobromobimane fluorescence intensity of the sperm tail was lower in normozoospermic than asthenozoospermic men, suggesting that thiol oxidation in spermatozoa of asthenozoospermic men is incomplete. Our findings indicate that mBBr affects the thiol status of ODF1 in human spermatozoa and interferes with progressive motility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Csiro Publishing
dc.rights
info:eu-repo/semantics/openAccess
dc.rights
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cytoskeleton
dc.subject
Epididymus
dc.subject
Fertility
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Thiols of flagellar proteins are essential for progressive motility in human spermatozoa
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-12T19:21:42Z
dc.journal.volume
29
dc.journal.number
7
dc.journal.pagination
1435-1446
dc.journal.pais
Australia
dc.journal.ciudad
Collingwood
dc.description.fil
Fil: Cabrillana, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina. Universidad del Aconcagua; Argentina
dc.description.fil
Fil: Monclus, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina. Universidad del Aconcagua; Argentina
dc.description.fil
Fil: Saez Lancellotti, Tania Emilce Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina. Universidad del Aconcagua; Argentina
dc.description.fil
Fil: Boarelli, Paola Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina. Universidad del Aconcagua; Argentina
dc.description.fil
Fil: Vincenti, Amanda Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina
dc.description.fil
Fil: Fornes, Miguel Matías. Universidad Nacional de Cuyo; Argentina
dc.description.fil
Fil: Sanabria, Eduardo Alfredo. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
dc.description.fil
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Histología y Embriología de Mendoza; Argentina. Universidad del Aconcagua; Argentina
dc.journal.title
Reproduction Fertility and Development
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1071/RD16225
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD16225
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