Artículo
Kinetics of Na+, K+-ATPase inhibition by an endogenous modulator (II- A)
Fecha de publicación:
02/2000
Editorial:
Springer/Plenum Publishers
Revista:
Neurochemical Research
ISSN:
0364-3190
e-ISSN:
1573-6903
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We have previously reported the isolation by gel filtration and anionic exchange HPLC of two brain Na+, K+-ATPase inhibitors, II-A and II-E, and kinetics of enzyme interaction with the latter. In the present study we evaluated the kinetics of synaptosomal membrane Na+, K+-ATPase with II-A and found that inhibitory activity was independent of ATP (2-8 mM), Na+ (3.1-100 mM), or K+ (2.5-40 mM) concentration. Hanes-Woolf plots showed that II-A decreases V(max) in all cases; K(M) value decreased for ATP but remained unaltered for Na+ and K+, indicating respectively uncompetitive and noncompetitive interaction. However, II-A became a stimulator at 0.3 mM K+ concentration. It is postulated that brain endogenous factor II-A may behave as a sodium pump modulator at the synaptic region, an action which depends on K+ concentration.
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Reines, Analia Gabriela; Peña, Clara; Rodriguez, Georgina Emma; Kinetics of Na+, K+-ATPase inhibition by an endogenous modulator (II- A); Springer/Plenum Publishers; Neurochemical Research; 25; 1; 2-2000; 121-127
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