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dc.contributor.author
Furmento, Verónica Alejandra
dc.contributor.author
Marino, Veronica Julieta
dc.contributor.author
Blank, Viviana Claudia
dc.contributor.author
Cayrol, Maria Florencia
dc.contributor.author
Cremaschi, Graciela Alicia
dc.contributor.author
Aguilar, Ruben Claudio
dc.contributor.author
Roguin, Leonor Patricia
dc.date.available
2018-03-19T17:23:50Z
dc.date.issued
2016-03
dc.identifier.citation
Furmento, Verónica Alejandra; Marino, Veronica Julieta; Blank, Viviana Claudia; Cayrol, Maria Florencia; Cremaschi, Graciela Alicia; et al.; Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation; Elsevier Inc; Experimental Cell Research; 342; 2; 3-2016; 125-134
dc.identifier.issn
0014-4827
dc.identifier.uri
http://hdl.handle.net/11336/39219
dc.description.abstract
Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of β1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1/2 and p38 MAPK. In addition, the increase of β1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates β1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Gcsf
dc.subject
Células Trofoblásticas
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Migración
dc.subject
Integrina
dc.subject.classification
Otras Ciencias Biológicas
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-15T14:06:11Z
dc.journal.volume
342
dc.journal.number
2
dc.journal.pagination
125-134
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Furmento, Verónica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Marino, Veronica Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Blank, Viviana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.description.fil
Fil: Cayrol, Maria Florencia. Pontificia Universidad Católica Argentina ; Argentina
dc.description.fil
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina ; Argentina
dc.description.fil
Fil: Aguilar, Ruben Claudio. Purdue University; Estados Unidos
dc.description.fil
Fil: Roguin, Leonor Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas ; Argentina
dc.journal.title
Experimental Cell Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.yexcr.2016.03.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S001448271630043X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26992288/


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