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dc.contributor.author
Lozada, Mariana  
dc.contributor.author
Rial, Daniela Veronica  
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Mac Cormack, Walter Patricio  
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Jansson, Janet K.  
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Sjöling, Sara  
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Carroll, JoLynn  
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Dionisi, Hebe Monica  
dc.date.available
2018-03-19T14:02:05Z  
dc.date.issued
2017-04  
dc.identifier.citation
Lozada, Mariana; Rial, Daniela Veronica; Mac Cormack, Walter Patricio; Jansson, Janet K.; Sjöling, Sara; et al.; Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: An in silico approach; Molecular Diversity Preservation International; Marine Drugs; 15; 4; 4-2017; 1-19  
dc.identifier.issn
1660-3397  
dc.identifier.uri
http://hdl.handle.net/11336/39169  
dc.description.abstract
The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer-Villiger and bacterial cytochrome P450 monooxygenases (CYP153). These enzymes have wide-ranging applications, from the synthesis of steroids, antibiotics, mycotoxins and pheromones to the synthesis of monomers for polymerization and anticancer precursors, due to their extraordinary enantio-, regio-, and chemo-selectivity that are valuable features for organic synthesis. Phylogenetic analyses were used to select the most divergent sequences affiliated to these enzyme families among the 264 putative monooxygenases recovered from the ~14 million protein-coding sequences in the assembled metagenome dataset. Three-dimensional structure modeling and docking analysis suggested features useful in biotechnological applications in five metagenomic sequences, such as wide substrate range, novel substrate specificity or regioselectivity. Further analysis revealed structural features associated with psychrophilic enzymes, such as broader substrate accessibility, larger catalytic pockets or low domain interactions, suggesting that they could be applied in biooxidations at room or low temperatures, saving costs inherent to energy consumption. This work allowed the identification of putative enzyme candidates with promising features from metagenomes, providing a suitable starting point for further developments.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Molecular Diversity Preservation International  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Bacterial Cytochrome P450  
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Baeyer-Villiger Monooxygenases  
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Bioprospecting Biocatalysts  
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Molecular Modeling  
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Phylogenetic Analysis  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Prospecting biotechnologically-relevant monooxygenases from cold sediment metagenomes: An in silico approach  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-03-02T15:11:52Z  
dc.journal.volume
15  
dc.journal.number
4  
dc.journal.pagination
1-19  
dc.journal.pais
Suiza  
dc.journal.ciudad
Basel  
dc.description.fil
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina  
dc.description.fil
Fil: Rial, Daniela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas Centro Científico Tecnológico - CONICET -Rosario. Instituto de Biologia Molecular y Celular de Rosario; Argentina  
dc.description.fil
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina  
dc.description.fil
Fil: Jansson, Janet K.. Pacific Northwest National Laboratory; Estados Unidos  
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Fil: Sjöling, Sara. Södertörn University; Suecia  
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Fil: Carroll, JoLynn. Fram-High North Research Centre for Climate and the Environment; Noruega. UiT The Arctic University of Norway; Noruega  
dc.description.fil
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina  
dc.journal.title
Marine Drugs  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1660-3397/15/4/114  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/md15040114