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dc.contributor.author
Battista, Daniela
dc.contributor.author
Ferrari, Carina Cintia
dc.contributor.author
Gage, Fred H.
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Pitossi, Fernando Juan
dc.date.available
2018-03-16T19:30:11Z
dc.date.issued
2006-01
dc.identifier.citation
Battista, Daniela; Ferrari, Carina Cintia; Gage, Fred H.; Pitossi, Fernando Juan; Neurogenic niche modulation by activated microglia: Transforming growth factor β increases neurogenesis in the adult dentate gyrus; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 23; 1; 1-2006; 83-93
dc.identifier.issn
0953-816X
dc.identifier.uri
http://hdl.handle.net/11336/39136
dc.description.abstract
Adult neural stem cells (NSC) proliferate and differentiate depending on the composition of the cellular and molecular niche in which they are immersed. Until recently, microglial cells have been ignored as part of the neurogenic niche. We studied the dynamics of NSC proliferation and differentiation in the dentate gyrus of the hippocampus (DG) and characterized the changes of the neurogenic niche in adrenalectomized animals (ADX). At the cellular level, we found increased NSC proliferation and neurogenesis in the ADX animals. In addition, a morphologically distinct subpopulation of NSC (Nestin+/GFAP-) with increased proliferating profile was detected. Interestingly, the number of microglial cells at stages 2 and 3 of activation correlated with increased neurogenesis (r2 = 0.999) and the number of Nestin-positive cells (r2 = 0.96). At the molecular level, transforming growth factor beta (TGF-β) mRNA levels were increased 10-fold in ADX animals. Interestingly, TGF-β levels correlated with the amount of neurogenesis detected (r 2 = 0.99) and the number of stage 2 and 3 microglial cells (r 2 = 0.94). Furthermore, blockade of TGF-β biological activity by administration of an anti-TGF-β type II receptor antibody diminished the percentage of 5-bromo-2′-deoxyuridine (BrdU)/q1PSA-NCAM-positive cells in vivo. Moreover, TGF-β was able to promote neurogenesis in NSC primary cultures. This work supports the idea that activated microglial cells are not pro- or anti-neurogenic per se, but the balance between pro- and anti-inflammatory secreted molecules influences the final effect of this activation. Importantly, we identified an anti-inflammatory cytokine, TGF-β, with neurogenic potential in the adult brain.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cytokine
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Inflammation
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Microglia
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Neuron
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Proliferation
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Stem Cells
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Neurogenic niche modulation by activated microglia: Transforming growth factor β increases neurogenesis in the adult dentate gyrus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-15T15:23:18Z
dc.identifier.eissn
1460-9568
dc.journal.volume
23
dc.journal.number
1
dc.journal.pagination
83-93
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Battista, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Ferrari, Carina Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Gage, Fred H.. Salk Institute for Biological Studies; Estados Unidos
dc.description.fil
Fil: Pitossi, Fernando Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
European Journal Of Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.2005.04539.x/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1460-9568.2005.04539.x


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