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dc.contributor.author Battista, Daniela
dc.contributor.author Ferrari, Carina Cintia
dc.contributor.author Gage, Fred H.
dc.contributor.author Pitossi, Fernando Juan
dc.date.available 2018-03-16T19:30:11Z
dc.date.issued 2006-01
dc.identifier.citation Battista, Daniela; Ferrari, Carina Cintia; Gage, Fred H.; Pitossi, Fernando Juan; Neurogenic niche modulation by activated microglia: Transforming growth factor β increases neurogenesis in the adult dentate gyrus; Wiley Blackwell Publishing, Inc; European Journal Of Neuroscience; 23; 1; 1-2006; 83-93
dc.identifier.issn 0953-816X
dc.identifier.uri http://hdl.handle.net/11336/39136
dc.description.abstract Adult neural stem cells (NSC) proliferate and differentiate depending on the composition of the cellular and molecular niche in which they are immersed. Until recently, microglial cells have been ignored as part of the neurogenic niche. We studied the dynamics of NSC proliferation and differentiation in the dentate gyrus of the hippocampus (DG) and characterized the changes of the neurogenic niche in adrenalectomized animals (ADX). At the cellular level, we found increased NSC proliferation and neurogenesis in the ADX animals. In addition, a morphologically distinct subpopulation of NSC (Nestin+/GFAP-) with increased proliferating profile was detected. Interestingly, the number of microglial cells at stages 2 and 3 of activation correlated with increased neurogenesis (r2 = 0.999) and the number of Nestin-positive cells (r2 = 0.96). At the molecular level, transforming growth factor beta (TGF-β) mRNA levels were increased 10-fold in ADX animals. Interestingly, TGF-β levels correlated with the amount of neurogenesis detected (r 2 = 0.99) and the number of stage 2 and 3 microglial cells (r 2 = 0.94). Furthermore, blockade of TGF-β biological activity by administration of an anti-TGF-β type II receptor antibody diminished the percentage of 5-bromo-2′-deoxyuridine (BrdU)/q1PSA-NCAM-positive cells in vivo. Moreover, TGF-β was able to promote neurogenesis in NSC primary cultures. This work supports the idea that activated microglial cells are not pro- or anti-neurogenic per se, but the balance between pro- and anti-inflammatory secreted molecules influences the final effect of this activation. Importantly, we identified an anti-inflammatory cytokine, TGF-β, with neurogenic potential in the adult brain.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley Blackwell Publishing, Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CYTOKINE
dc.subject INFLAMMATION
dc.subject MICROGLIA
dc.subject NEURON
dc.subject PROLIFERATION
dc.subject STEM CELLS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Neurogenic niche modulation by activated microglia: Transforming growth factor β increases neurogenesis in the adult dentate gyrus
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-03-15T15:23:18Z
dc.identifier.eissn 1460-9568
dc.journal.volume 23
dc.journal.number 1
dc.journal.pagination 83-93
dc.journal.pais Reino Unido
dc.journal.ciudad Oxford
dc.description.fil Fil: Battista, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Ferrari, Carina Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Gage, Fred H.. Salk Institute for Biological Studies; Estados Unidos
dc.description.fil Fil: Pitossi, Fernando Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title European Journal Of Neuroscience
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.2005.04539.x/full
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1460-9568.2005.04539.x
dc.conicet.fuente unificacion


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)