Artículo
Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging
Lores Arnaiz, Silvia
; Lombardi, Paulina; Karadayian, Analia Graciela
; Orgambide, Federico Oscar; Cicerchia, Daniela Griselda; Bustamante, Juanita
Fecha de publicación:
01/2016
Editorial:
Springer/Plenum Publishers
Revista:
Neurochemical Research
ISSN:
0364-3190
e-ISSN:
1573-6903
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evaluate the susceptibility of brain cortex synaptosomes and non-synaptic mitochondria to aging-dependent dysfunction, male Swiss mice of 3 or 17 months old were used. Mitochondrial function was evaluated by oxygen consumption, mitochondrial membrane potential and respiratory complexes activity, together with UCP-2 protein expression. Basal respiration and respiration driving proton leak were decreased by 26 and 33 % in synaptosomes from 17-months old mice, but spare respiratory capacity was not modified by aging. Succinate supported state 3 respiratory rate was decreased by 45 % in brain cortex non-synaptic mitochondria from 17-month-old mice, as compared with young animals, but respiratory control was not affected. Synaptosomal mitochondria would be susceptible to undergo calcium-induced depolarization in 17 months-old mice, while non-synaptic mitochondria would not be affected by calcium overload. UCP-2 was significantly upregulated in both synaptosomal and submitochondrial membranes from 17-months old mice, compared to young animals. UCP-2 upregulation seems to be a possible mechanism by which mitochondria would be resistant to suffer oxidative damage during aging.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBIMOL)
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Citación
Lores Arnaiz, Silvia; Lombardi, Paulina; Karadayian, Analia Graciela; Orgambide, Federico Oscar; Cicerchia, Daniela Griselda; et al.; Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging; Springer/Plenum Publishers; Neurochemical Research; 41; 1-2; 1-2016; 353-363
Compartir
Altmétricas