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Artículo

Dermatan sulfate/chitosan polyelectrolyte complex with potential application in the treatment and diagnosis of vascular disease

Rasente, Rita Yanina; Imperiale, Julieta CelesteIcon ; Lazaro Martinez, Juan ManuelIcon ; Gualco, Luciana; Oberkersch, Roxana ElenaIcon ; Sosnik, Alejandro DarioIcon ; Calabrese, Graciela Cristina
Fecha de publicación: 06/2016
Editorial: Elsevier
Revista: Carbohydrate Polymers
ISSN: 0144-8617
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Cardiovascular disease is the largest single cause of morbid-mortality in the world. However, there is still no pharmaceutical treatment that directly targets the blood vessel wall instead of just controlling the risk factors. Here, we produced polyelectrolyte complexes (PECs) by a simple and reproducible polyelectrolyte complexation method between low molecular mass dermatan sulfate (polyanionic polysaccharide) and chitosan (polycationic polysaccharide), and evaluated the cellular uptake by vascular endothelial cells. The composition and the composition homogeneity of PECs were confirmed by 13C-CP-MAS spectroscopy and by polyacrylamide gel electrophoresis, respectively. The hydrodynamic radius, determined by dynamic light scattering, was 729 ± 11 nm. PECs were not cytotoxic for a murine heart endothelium-derived cell line. Fluorescent confocal microscopy showed the specific uptake of fluorescently-labeled PECs by endothelial cells when they were cultured alone or in the presence of macrophages. Overall, these findings confirmed the potential of these PECs for targeting different agents to the vessel wall in the prevention, diagnosis, and therapy of vascular disease.
Palabras clave: Chitosan , Endothelial Uptake , Low Molecular Mass Dermatan Sulfate , Polyelectrolyte Complexes , Vascular Diseasea
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/38955
DOI: http://dx.doi.org/10.1016/j.carbpol.2016.02.046
URL: https://www.sciencedirect.com/science/article/pii/S0144861716301114
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Rasente, Rita Yanina; Imperiale, Julieta Celeste; Lazaro Martinez, Juan Manuel; Gualco, Luciana; Oberkersch, Roxana Elena; et al.; Dermatan sulfate/chitosan polyelectrolyte complex with potential application in the treatment and diagnosis of vascular disease; Elsevier; Carbohydrate Polymers; 144; 6-2016; 362-370
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