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dc.contributor.author
Dlugovitzky, Diana
dc.contributor.author
Fontela, María Sol
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Martinel Lamas, Diego José
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Valdez, Ricardo A.
dc.contributor.author
Romano, Marta C.
dc.date.available
2018-03-14T20:18:27Z
dc.date.issued
2015-04
dc.identifier.citation
Dlugovitzky, Diana; Fontela, María Sol; Martinel Lamas, Diego José; Valdez, Ricardo A.; Romano, Marta C.; Mycobacterium smegmatis synthesizes in vitro androgens and estrogens from different steroid precursors; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 61; 7; 4-2015; 451-455
dc.identifier.issn
0008-4166
dc.identifier.uri
http://hdl.handle.net/11336/38804
dc.description.abstract
Fast-growing mycobacteria such as Mycobacterium sp. and Mycobacterium smegmatis degrade natural sterols. They are a model to study tuberculosis. Interestingly, M. smegmatis has been found in river effluents derived from paper production, and therefore, it would be important to gain further insight into its capacity to synthesize steroids that are potential endocrine disruptors affecting the development and reproduction of fishes. To our knowledge, the capacity of M. smegmatis to synthesize estrogens and even testosterone has not been previously reported. Therefore, the objective of this study was to investigate the capacity of M. smegmatis to synthesize in vitro testosterone and estrogens from tritiated precursors and to investigate the metabolic pathways involved. Results obtained by thin-layer chromatography showed that3H-progesterone was transformed to 17OH-progesterone, androstenedione, testosterone, estrone, and estradiol after 6, 12, or 24 h of incubation.3H-androstenedione was transformed into testosterone and estrogens, mainly estrone, and3H-testosterone was transformed to estrone and andro-stenedione. Incubation with3H-dehydroepiandrosterone rendered androstenediol, testosterone, and estrogens. This ability to transform less potent sex steroids like androstenedione and estrone into other more active steroids like testosterone and estradiol or vice versa suggests that M. smegmatis can influence the amount of self-synthesized strong androgens and estrogens and can transform those found in the environment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Research Council Canada-NRC Research Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Androgens
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Estrogens
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Mycobacterium
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Mycobacterium Smegmatis
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Steroid Synthesis
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Mycobacterium smegmatis synthesizes in vitro androgens and estrogens from different steroid precursors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-13T13:58:09Z
dc.journal.volume
61
dc.journal.number
7
dc.journal.pagination
451-455
dc.journal.pais
Canadá
dc.journal.ciudad
Otawa
dc.description.fil
Fil: Dlugovitzky, Diana. Universidad Nacional de Rosario; Argentina
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Fil: Fontela, María Sol. Universidad Nacional de Rosario; Argentina
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Fil: Martinel Lamas, Diego José. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Valdez, Ricardo A.. Instituto Politécnico Nacional; México
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Fil: Romano, Marta C.. Instituto Politécnico Nacional; México
dc.journal.title
Canadian Journal Of Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2015-0025?journalCode=cjm#.WqldPPnOWJA
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1139/cjm-2015-0025
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