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dc.contributor.author
Daniel, María Alejandra
dc.contributor.author
Barrionuevo, Matías Raul
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Doyle, Santiago Raúl
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Vullo, Diana Lia
dc.date.available
2018-03-09T20:06:20Z
dc.date.issued
2016-01
dc.identifier.citation
Daniel, María Alejandra; Barrionuevo, Matías Raul; Doyle, Santiago Raúl; Vullo, Diana Lia; Kinetics of Pseudomonas veronii 2E biofilm development under different nutritional conditions for a proper bioreactor design; Elsevier Science Sa; Biochemical Engineering Journal; 105; 1-2016; 150-158
dc.identifier.issn
1369-703X
dc.identifier.uri
http://hdl.handle.net/11336/38470
dc.description.abstract
Biofilm-mediated bioreactors represent a proficient alternative, as extracellular materials of bacterial biofilms enhance metal immobilization by biosorption. Bacterial attachment or detachment is related to environmental conditions. The aim of this work is to study the nutritional dependence of Pseudomonas veronii 2E biofilm development to be applied in a future biofilm-reactor for wastewater treatments. For such purpose, biofilm establishment kinetics was explored over glass coverslips changing medium composition and nutrient concentration and in Fe(II) presence. After crystal violet cell staining, biofilm was visualized using direct microscopic observation; sample scanning-image analysis implemented in MATLAB and ethanol extraction-Absorbance 590. nm measurement. Biofilm structure developed with mineral basal medium appeared as a monolayer, but in complex basal media cell aggregates were appreciated. Optimal attachment was achieved with complex basal medium-5. g/L glucose at 55. h. The crystal violet concentration complemented with the image capturing supported these results. Biofilm establishment was enhanced by biosurfactant production in P. veronii 2E. The obtention of mutants deficient in biosurfactant secretion confirmed the influence of these compounds on cell motility and hence on cell attachment. The information of the biofilm structure defined under such conditions is the useful first step for the future bioreactor design.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Sa
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Biofilm Reactor
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Biofilm Stability
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Biosorption
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Metal Bioremediation
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Pseudomonas Veronii
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Wastewater Biotreatment
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Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
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Biotecnología del Medio Ambiente
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Kinetics of Pseudomonas veronii 2E biofilm development under different nutritional conditions for a proper bioreactor design
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-09T18:58:04Z
dc.journal.volume
105
dc.journal.pagination
150-158
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Daniel, María Alejandra. Universidad Nacional de General Sarmiento. Instituto de Ciencias. Área de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Barrionuevo, Matías Raul. Universidad Nacional de General Sarmiento. Instituto de Ciencias. Área de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Doyle, Santiago Raúl. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vullo, Diana Lia. Universidad Nacional de General Sarmiento. Instituto de Ciencias. Área de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Biochemical Engineering Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bej.2015.09.001
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X15300462
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