Artículo
Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization
Fecha de publicación:
12/2008
Editorial:
Sociedade Brasileira de Genética
Revista:
Genetics and Molecular Biology
ISSN:
1415-4757
e-ISSN:
1678-4685
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome) of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results indicating that the D genome is positioned between the A and B genomes
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Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Robledo Dobladez, Germán Ariel; Seijo, José Guillermo; Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization; Sociedade Brasileira de Genética; Genetics and Molecular Biology; 31; 3; 12-2008; 717-724
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