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dc.contributor.author
Daloso, Danilo M.
dc.contributor.author
Müller, Karolin
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Obata, Toshihiro
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Florian, Alexandra
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Tohge, Takayuki
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Bottcher, Alexandra
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Riondet, Christophe
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Bariat, Laetitia
dc.contributor.author
Carrari, Fernando Oscar
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Nunes Nesi, Adriano
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Buchanan, Bob B.
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Reichheld, Jean-Philippe
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Araújo, Wagner L.
dc.contributor.author
Fernie, Alisdair R.
dc.date.available
2018-03-09T19:25:33Z
dc.date.issued
2015-03
dc.identifier.citation
Daloso, Danilo M.; Müller, Karolin; Obata, Toshihiro; Florian, Alexandra; Tohge, Takayuki; et al.; Thioredoxin, a master regulator of the tricarboxylic acid cycle in plant mitochondria; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 112; 11; 3-2015; E1392-E1400
dc.identifier.issn
0027-8424
dc.identifier.uri
http://hdl.handle.net/11336/38435
dc.description.abstract
Plant mitochondria have a fully operational tricarboxylic acid (TCA) cycle that plays a central role in generating ATP and providing carbon skeletons for a range of biosynthetic processes in both heterotrophic and photosynthetic tissues. The cycle enzyme-encoding genes have been well characterized in terms of transcriptional and effector-mediated regulation and have also been subjected to reverse genetic analysis. However, despite this wealth of attention, a central question remains unanswered: "What regulates flux through this pathway in vivo?" Previous proteomic experiments with Arabidopsis discussed below have revealed that a number of mitochondrial enzymes, including members of the TCA cycle and affiliated pathways, harbor thioredoxin (TRX)-binding sites and are potentially redox-regulated. We have followed up on this possibility and found TRX to be a redox-sensitive mediator of TCA cycle flux. In this investigation, we first characterized, at the enzyme and metabolite levels, mutants of the mitochondrial TRX pathway in Arabidopsis: the NADP-TRX reductase a and b double mutant (ntra ntrb) and the mitochondrially located thioredoxin o1 (trxo1) mutant. These studies were followed by a comparative evaluation of the redistribution of isotopes when 13C-glucose, 13C-malate, or 13C-pyruvate was provided as a substrate to leaves of mutant or WT plants. In a complementary approach, we evaluated the in vitro activities of a range of TCA cycle and associated enzymes under varying redox states. The combined dataset suggests that TRX may deactivate both mitochondrial succinate dehydrogenase and fumarase and activate the cytosolic ATP-citrate lyase in vivo, acting as a direct regulator of carbon flow through the TCA cycle and providing a mechanism for the coordination of cellular function.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Academy of Sciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arabidopsis
dc.subject
Atp-Citrate Lyase
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Citric Acid Cycle Regulation
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Redox Regulation
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Thioredoxin Tca Cycle Regulation
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Thioredoxin, a master regulator of the tricarboxylic acid cycle in plant mitochondria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-08T19:02:43Z
dc.journal.volume
112
dc.journal.number
11
dc.journal.pagination
E1392-E1400
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Daloso, Danilo M.. Institut Max Planck Fur Molekulare Physiologie; Alemania. Universidade Federal de Vicosa; Brasil
dc.description.fil
Fil: Müller, Karolin. Institut Max Planck Fur Molekulare Physiologie; Alemania
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Fil: Obata, Toshihiro. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Florian, Alexandra. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Tohge, Takayuki. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Bottcher, Alexandra. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Riondet, Christophe. Centre National de la Recherche Scientifique; Francia
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Fil: Bariat, Laetitia. Centre National de la Recherche Scientifique; Francia
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Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
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Fil: Nunes Nesi, Adriano. Universidade Federal de Vicosa; Brasil
dc.description.fil
Fil: Buchanan, Bob B.. University of California at Berkeley; Estados Unidos
dc.description.fil
Fil: Reichheld, Jean-Philippe. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Araújo, Wagner L.. Universidade Federal de Vicosa; Brasil
dc.description.fil
Fil: Fernie, Alisdair R.. Institut Max Planck Fur Molekulare Physiologie; Alemania
dc.journal.title
Proceedings of the National Academy of Sciences of The United States of America
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/112/11/E1392.short
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1073/pnas.1424840112
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