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dc.contributor.author
Sydes, Daniel
dc.contributor.author
Kler, Pablo Alejandro
dc.contributor.author
Meyer, Hans
dc.contributor.author
Zipfl, Peter
dc.contributor.author
Lutz, Daniel
dc.contributor.author
Huhn, Carolin
dc.date.available
2018-03-08T19:19:40Z
dc.date.issued
2016-12
dc.identifier.citation
Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-8725
dc.identifier.issn
1618-2642
dc.identifier.uri
http://hdl.handle.net/11336/38288
dc.description.abstract
Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Glass Microchip
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Isoelectric Focusing
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On-Chip Led-If Detection
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Two-Dimensional Separations
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Voltage Switching
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Ingeniería de Sistemas y Comunicaciones
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Ingeniería Eléctrica, Ingeniería Electrónica e Ingeniería de la Información
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-07T15:53:58Z
dc.identifier.eissn
1618-2650
dc.journal.volume
408
dc.journal.number
30
dc.journal.pagination
8713-8725
dc.journal.pais
Alemania
dc.journal.ciudad
Heidelberg
dc.description.fil
Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; Alemania
dc.description.fil
Fil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
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Fil: Meyer, Hans. J&M Analytik AG; Alemania
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Fil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; Alemania
dc.description.fil
Fil: Lutz, Daniel. CalvaSens GmbH; Alemania
dc.description.fil
Fil: Huhn, Carolin. Eberhard Karls Universität Tübingen; Alemania
dc.journal.title
Analytical and Bioanalytical Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00216-016-0033-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00216-016-0033-8
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