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dc.contributor.author Panero, Julieta
dc.contributor.author O'Callaghan, Nathan J.
dc.contributor.author Fenech, Michael
dc.contributor.author Slavutsky, Irma Rosa
dc.date.available 2018-03-08T15:50:41Z
dc.date.issued 2015-02
dc.identifier.citation Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa; Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders; Humana Press; Molecular Biotechnology; 57; 2; 2-2015; 155-159
dc.identifier.issn 1073-6085
dc.identifier.uri http://hdl.handle.net/11336/38264
dc.description.abstract Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.
dc.format application/pdf
dc.language.iso eng
dc.publisher Humana Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ABSOLUTE QUANTIFICATION
dc.subject BONE MARROW SAMPLES
dc.subject MGUS
dc.subject MULTIPLE MYELOMA
dc.subject TELOMERE LENGTH
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-03-06T17:44:15Z
dc.journal.volume 57
dc.journal.number 2
dc.journal.pagination 155-159
dc.journal.pais Estados Unidos
dc.journal.ciudad Oregon
dc.description.fil Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil Fil: O'Callaghan, Nathan J.. Commonwealth Scientific and Industrial Research Organization; Australia
dc.description.fil Fil: Fenech, Michael. Commonwealth Scientific and Industrial Research Organization; Australia
dc.description.fil Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.journal.title Molecular Biotechnology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12033-014-9811-8
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12033-014-9811-8
dc.conicet.fuente individual


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)