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dc.contributor.author
Panero, Julieta
dc.contributor.author
O'Callaghan, Nathan J.
dc.contributor.author
Fenech, Michael
dc.contributor.author
Slavutsky, Irma Rosa
dc.date.available
2018-03-08T15:50:41Z
dc.date.issued
2015-02
dc.identifier.citation
Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa; Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders; Humana Press; Molecular Biotechnology; 57; 2; 2-2015; 155-159
dc.identifier.issn
1073-6085
dc.identifier.uri
http://hdl.handle.net/11336/38264
dc.description.abstract
Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Absolute Quantification
dc.subject
Bone Marrow Samples
dc.subject
Mgus
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Multiple Myeloma
dc.subject
Telomere Length
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-06T17:44:15Z
dc.journal.volume
57
dc.journal.number
2
dc.journal.pagination
155-159
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Oregon
dc.description.fil
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: O'Callaghan, Nathan J.. Commonwealth Scientific and Industrial Research Organization; Australia
dc.description.fil
Fil: Fenech, Michael. Commonwealth Scientific and Industrial Research Organization; Australia
dc.description.fil
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.journal.title
Molecular Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12033-014-9811-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12033-014-9811-8
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