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dc.contributor.author
Magnoni, Leonardo Julián
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dc.contributor.author
Roher, Nerea
dc.contributor.author
Crespo, Diego
dc.contributor.author
Krasnov, Aleksei
dc.contributor.author
Planas, Josep V.
dc.date.available
2018-03-07T19:40:39Z
dc.date.issued
2015-02
dc.identifier.citation
Magnoni, Leonardo Julián; Roher, Nerea; Crespo, Diego; Krasnov, Aleksei; Planas, Josep V.; In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss); MDPI AG; Biology; 4; 1; 2-2015; 67-87
dc.identifier.issn
2079-7737
dc.identifier.uri
http://hdl.handle.net/11336/38177
dc.description.abstract
The physiological consequences of the activation of the immune system in skeletal muscle in fish are not completely understood. To study the consequences of the activation of the immune system by bacterial pathogens on skeletal muscle function, we administered lipopolysaccharide (LPS), an active component of Gram-negative bacteria, in rainbow trout and performed transcriptomic and proteomic analyses in skeletal muscle. We examined changes in gene expression in fast and slow skeletal muscle in rainbow trout at 24 and 72 h after LPS treatment (8 mg/kg) by microarray analysis. At the transcriptional level, we observed important changes in metabolic, mitochondrial and structural genes in fast and slow skeletal muscle. In slow skeletal muscle, LPS caused marked changes in the expression of genes related to oxidative phosphorylation, while in fast skeletal muscle LPS administration caused major changes in the expression of genes coding for glycolytic enzymes. We also evaluated the effects of LPS administration on the fast skeletal muscle proteome and identified 14 proteins that were differentially induced in LPS-treated trout, primarily corresponding to glycolytic enzymes. Our results evidence a robust and tissue-specific response of skeletal muscle to an acute inflammatory challenge, affecting energy utilization and possibly growth in rainbow trout.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI AG
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fish
dc.subject
Lipopolysaccharide
dc.subject
Muscle
dc.subject
Proteome
dc.subject
Transcriptome
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
In vivo molecular responses of fast and slow muscle fibers to Lipopolysaccharide in a Teleost fish, the rainbow trout (Oncorhynchus mykiss)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-06T17:44:01Z
dc.journal.volume
4
dc.journal.number
1
dc.journal.pagination
67-87
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: Magnoni, Leonardo Julián. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Roher, Nerea. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Crespo, Diego. Universidad de Barcelona; España
dc.description.fil
Fil: Krasnov, Aleksei. Nofima; Noruega
dc.description.fil
Fil: Planas, Josep V.. Universidad de Barcelona; España
dc.journal.title
Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/biology4010067
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/2079-7737/4/1/67
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