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dc.contributor.author
Mareze, Vania Aparecida
dc.contributor.author
Cristina, Borio
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Bilen, Marcos Fabian
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Fleith, Renata
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Mirazo, Santiago
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Santos Mansur, Daniel
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Bruña Romero, Oscar
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Arbiza, Juan
dc.contributor.author
Mario Enrique, Lozano
dc.date.available
2016-01-26T14:50:33Z
dc.date.issued
2015-09-19
dc.identifier.citation
Mareze, Vania Aparecida; Cristina, Borio; Bilen, Marcos Fabian; Fleith, Renata; Mirazo, Santiago; et al.; Tests in mice of a dengue vaccine candidate made of chimeric Junin virus-like particles and conserved dengue virus envelope sequences; Springer; Applied Microbiology And Biotechnology; 19-9-2015
dc.identifier.issn
0175-7598
dc.identifier.uri
http://hdl.handle.net/11336/3815
dc.description.abstract
Two new vaccine candidates against dengue virus (DENV) infection were generated by fusing the coding sequences of the self-budding Z protein from Junin virus (Z-JUNV) to those of two cryptic peptides (Z/DENV-P1 and Z/DENV-P2) conserved on the envelope protein of all serotypes of DENV. The capacity of these chimeras to generate virus-like particles (VLPs) and to induce virus-neutralizing antibodies in mice was determined. First, recombinant proteins that displayed reactivity with a Z-JUNV-specific serum by immunofluorescence were detected in HEK-293 cells transfected with each of the two plasmids and VLP formation was also observed by transmission electron microscopy. Next, we determined the presence of antibodies against the envelope peptides of DENV in the sera of immunized C57BL/6 mice. Results showed that those animals that received Z/DENV-P2 DNA coding sequences followed by a boost with DENV-P2 synthetic peptides elicited significant specific antibody titers (≥6.400). Finally, DENV plaque-reduction neutralization tests (PRNT) were performed. Although no significant protective effect was observed when using sera of Z/DENV-P1-immunized animals, antibodies raised against vaccine candidate Z/DENV-P2 (diluted 1:320) were able to reduce in over 50 % the number of viral plaques generated by infectious DENV particles. This reduction was comparable to that of the 4G2 DENV-specific monoclonal cross-reactive (all serotypes) neutralizing antibody. We conclude that Z-JUNV-VLP is a valid carrier to induce antibody-mediated immune responses in mice and that Z/DENV-P2 is not only immunogenic but also protective in vitro against infection of cells with DENV, deserving further studies. On the other side, DENV´s fusion peptide-derived chimera Z/DENV-P1 did not display similar protective properties
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Vlps
dc.subject
Junin
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Dengue
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Vaccine
dc.subject.classification
Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar
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Biotecnología de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Tests in mice of a dengue vaccine candidate made of chimeric Junin virus-like particles and conserved dengue virus envelope sequences
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Mareze, Vania Aparecida. 1UNIVERSIDADE FEDERAL DE SANTA CATARINA;
dc.description.fil
Fil: Cristina, Borio. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; Argentina
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Fil: Bilen, Marcos Fabian. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; Argentina
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Fil: Fleith, Renata. Universidade Federal Da Santa Catarina; Brasil
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Fil: Mirazo, Santiago. Universidad de la Republica; Uruguay
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Fil: Santos Mansur, Daniel. Universidade Federal Da Santa Catarina; Brasil
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Fil: Bruña Romero, Oscar. Universidade Federal Da Santa Catarina; Brasil
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Fil: Arbiza, Juan. Universidad de la Republica; Uruguay
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Fil: Mario Enrique, Lozano. Universidad Nacional de Quilmes; Argentina
dc.journal.title
Applied Microbiology And Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00253-015-6973-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http:dx.doi.org/10.1007/s00253-015-6973-7
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