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dc.contributor.author
Livore, Veronica Ines
dc.contributor.author
Uttaro, Antonio Domingo
dc.date.available
2018-03-06T18:12:44Z
dc.date.issued
2015-01
dc.identifier.citation
Livore, Veronica Ines; Uttaro, Antonio Domingo; Biosynthesis of very long chain fatty acids in Trypanosoma cruzi; Springer; Parasitology Research; 114; 1; 1-2015; 265-271
dc.identifier.issn
0932-0113
dc.identifier.uri
http://hdl.handle.net/11336/37971
dc.description.abstract
Trypanosoma brucei and Trypanosoma cruzi showed similar fatty acid (FA) compositions, having a high proportion of unsaturated FAs, mainly 18:2Δ9,12 (23–39 %) and 18:1Δ9 (11–17 %). C22 polyunsaturated FAs are in significant amounts only in T. brucei (12–20 %) but represent a mere 2 % of total FAs in T. cruzi. Both species have also similar profiles of medium- and long-chain saturated FAs, from 14:0 to 20:0. Interestingly, procyclic and bloodstream forms of T. brucei lack very long chain FAs (VLCFAs), whereas epimastigotes and trypomastigotes of T. cruzi contain 22:0 (0.1–0.2 %), 24:0 (1.5–2 %), and 26:0 (0.1–0.2 %). This is in agreement with the presence of an additional FA elongase gene (TcELO4) in T. cruzi. TcELO4 was expressed in a Saccharomyces cerevisiae mutant lacking the endogenous ScELO3, rescuing the synthesis of saturated and hydroxylated C26 FAs in the yeast. Expression of TcELO4 also rescued the synthetic lethality of a ScELO2, ScELO3 double mutation, and the VLCFA profile of the transformed yeast was similar to that found in T. cruzi. By identifying TcELO4 as the enzyme responsible for the elongation of FA from 16:0 and 18:0 up to 26:0, with 24:0 being the preferred product, this work completed the characterization of FA elongases in Trypanosoma spp.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Elongase
dc.subject
Lipids
dc.subject
Trypanosoma Cruzi
dc.subject
Very Long Chain Fatty Acid
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Biosynthesis of very long chain fatty acids in Trypanosoma cruzi
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-02T14:22:22Z
dc.identifier.eissn
1432-1955
dc.journal.volume
114
dc.journal.number
1
dc.journal.pagination
265-271
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Livore, Veronica Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Uttaro, Antonio Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title
Parasitology Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00436-014-4188-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00436-014-4188-y


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