Mostrar el registro sencillo del ítem

dc.contributor.author
Leccese Terraf, Maria Cecilia
dc.contributor.author
Juárez Tomás, María Silvina
dc.contributor.author
Rault, Lucie
dc.contributor.author
Le Loir, Yves
dc.contributor.author
Even, Sergine
dc.contributor.author
Nader, Maria Elena Fatima
dc.date.available
2018-03-05T19:26:35Z
dc.date.issued
2016-09
dc.identifier.citation
Leccese Terraf, Maria Cecilia; Juárez Tomás, María Silvina; Rault, Lucie; Le Loir, Yves; Even, Sergine; et al.; Biofilms of vaginal Lactobacillus reuteri CRL 1324 and Lactobacillus rhamnosus CRL 1332: kinetics of formation and matrix characterization; Springer; Archives of Microbiology; 198; 7; 9-2016; 689-700
dc.identifier.issn
0302-8933
dc.identifier.uri
http://hdl.handle.net/11336/37872
dc.description.abstract
Adhesion and biofilm formation are strain properties that reportedly contribute to the permanence of lactobacilli in the human vagina. The kinetics of biofilm formation and the chemical nature of the biofilm matrix formed by Lactobacillus reuteri CRL (Centro de Referencia para Lactobacilos Culture Collection) 1324 and Lactobacillus rhamnosus CRL 1332, vaginal beneficial strains, were evaluated in this work. Crystal violet-stained microplate assay and techniques of epifluorescence, electron and confocal microscopy were applied. The highest density and complexity of biofilms of both vaginal lactobacilli were observed at 72 h of incubation. Protease, proteinase K, α-chymotrypsin and trypsin treatments efficiently detached L. reuteri CRL 1324 biofilm that was also partially affected by α-amylase. However, L. rhamnosus CRL 1332 biofilm was slightly affected by protease, proteinase K and α-amylase. Confocal microscopy revealed greater amount of polysaccharides in L. rhamnosus CRL 1332 biofilm matrix than in L. reuteri CRL 1324 biofilm matrix. The results indicate that proteins are one of the main components of the L. reuteri CRL 1324 biofilm, while the biofilm matrix of L. rhamnosus CRL 1332 is composed of carbohydrates and proteins. The results obtained support the knowledge, understanding and characterization of two biofilm-forming vaginal Lactobacillus strains.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Beneficial Microorganisms
dc.subject
Biofilm Formation
dc.subject
Biofilm Matrix
dc.subject
Confocal Microscopy
dc.subject
Probiotics
dc.subject
Vaginal Lactobacilli
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Biofilms of vaginal Lactobacillus reuteri CRL 1324 and Lactobacillus rhamnosus CRL 1332: kinetics of formation and matrix characterization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-02T15:11:23Z
dc.identifier.eissn
1432-072X
dc.journal.volume
198
dc.journal.number
7
dc.journal.pagination
689-700
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Rault, Lucie. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Le Loir, Yves. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Even, Sergine. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.journal.title
Archives of Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00203-016-1225-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00203-016-1225-5