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Artículo

Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm

Pelufo, ValeriaIcon ; Lopez Armengol, Maria FernandaIcon ; Malcotti, Valeria; Venturino, AndresIcon ; Aisen, Eduardo Gabriel
Fecha de publicación: 01/2015
Editorial: Elsevier Science Inc
Revista: Theriogenology
ISSN: 0093-691X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnología Agropecuaria

Resumen

Sperm deep freezing procedures for ram semen have considerable variations regarding the steps being employed for cooling, freezing, and addition of cryoprotectants. In this work, we evaluated the effects of the addition of glycerol and/or the disaccharides sucrose and trehalose to hypertonic diluents either before or after cooling from 30°C to 5°C in Merino Australian ram semen cryopreservation. Using optical and transmission electron microscopy techniques, we assessed that glycerol was beneficial to the cooling process independently of its addition at 30°C or 5°C in terms of sperm membrane integrity in different regions of the plasma membrane (acrosomal region, 14.5% higher integrity; postacrosomal region, 8.0% higher integrity [P<0.01]; hypoosmotic swelling test [HOST], 10.8% higher integrity [P<0.001]). Disaccharides were necessary for a better cryopreservation in liquid nitrogen, and the best procedure was their addition after cooling at 5°C (12% higher sperm motility [P<0.001]; 8% higher acrosome integrity, [P<0.05]; 9.5% higher plasma membrane integrity assessed by HOST [P<0.001]). Trehalose showed a greater preservation cryoprotectant capacity than sucrose, as indicated by sperm motility after thawing (8.1% greater [P<0.01]) and by the integrity of the intermediate piece (20% greater [P<0.05]). From these results, we conclude that the best procedure for ram semen cryopreservation in hypertonic disaccharide-containing diluents is the addition of glycerol and trehalose after the cooling process, at 5°C.
Palabras clave: Cooling Process , Frozen-Thawed Ram Spermatozoa , Plasma Membrane Domains , Trehalose
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/37853
DOI: http://dx.doi.org/10.1016/j.theriogenology.2014.09.007
URL: https://www.sciencedirect.com/science/article/pii/S0093691X14004968
Colecciones
Articulos(CCT - PATAGONIA NORTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA NORTE
Articulos(PROBIEN)
Articulos de INST. DE INVESTIGACION Y DES. EN ING. DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Citación
Pelufo, Valeria; Lopez Armengol, Maria Fernanda; Malcotti, Valeria; Venturino, Andres; Aisen, Eduardo Gabriel; Effects of glycerol and sugar mixing temperature on the morphologic and functional integrity of cryopreserved ram sperm; Elsevier Science Inc; Theriogenology; 83; 1; 1-2015; 144-151
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