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dc.contributor.author
Qi, Yulin
dc.contributor.author
Rosso, Lorena Vanesa
dc.contributor.author
Sedan, Daniela Yazmine
dc.contributor.author
Giannuzzi, Leda
dc.contributor.author
Andrinolo, Dario
dc.contributor.author
Volmer, Dietrich A.
dc.date.available
2018-03-05T19:12:49Z
dc.date.issued
2015-01
dc.identifier.citation
Qi, Yulin; Rosso, Lorena Vanesa; Sedan, Daniela Yazmine; Giannuzzi, Leda; Andrinolo, Dario; et al.; Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - Unambiguous assignment of product ions by tandem mass spectrometry; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry ; 29; 2; 1-2015; 220-224
dc.identifier.issn
0951-4198
dc.identifier.uri
http://hdl.handle.net/11336/37840
dc.description.abstract
RATIONALE High-resolution mass spectrometry was applied to the study of a Microcystis aeruginosa strain previously reported as a [D-Leu1]MC-LR producer. Detailed analysis revealed new microcystin (MC) variants produced from the strain, and seven of these were previously unreported variants. This work shows the importance of mass accuracy for the identification of unknown MCs. METHODS The M. aeruginosa strain was isolated from a bloom sample collected from Argentina and acclimated to lab conditions. The MC variants in the strain were separated by UV/Vis detection-guided high-performance liquid chromatography, and their structures were unambiguous determined by tandem mass spectrometry (MS/MS). RESULTS A simple strategy was developed for quickly locating the low-abundance MC precursors from complex samples. MS/MS anlysis revealed ten MC variants produced from the strain, of which seven have never been reported before. CONCLUSIONS This work shows the interference of isobarics and isomers in the study of unknown MCs, and, therefore, high mass accuracy is important to avoid false assignments. Moreover, the peak list provided here (30-50 fragments unambiguously assigned for ten MCs) can be used as a reference for the discovery of MCs from environmental samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ne Microcystins
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Ms-Ms Detection
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Microcystin Aeruginosa Strain
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - Unambiguous assignment of product ions by tandem mass spectrometry
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-02T13:58:44Z
dc.journal.volume
29
dc.journal.number
2
dc.journal.pagination
220-224
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Qi, Yulin. Universitat Saarland; Alemania
dc.description.fil
Fil: Rosso, Lorena Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Sedan, Daniela Yazmine. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Giannuzzi, Leda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Andrinolo, Dario. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Volmer, Dietrich A.. Universitat Saarland; Alemania
dc.journal.title
Rapid Communications in Mass Spectrometry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/rcm.7098
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/rcm.7098/abstract


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