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Artículo

Ca2+ Sparks and Ca2+ waves are the subcellular events underlying Ca2+ overload during ischemia and reperfusion in perfused intact hearts

Mattiazzi, Ramona AliciaIcon ; Argenziano, Mariana AlejandraIcon ; Aguilar-Sanchez, Yuriana; Mazzocchi, GabrielaIcon ; Escobar, Ariel L.
Fecha de publicación: 02/2015
Editorial: Academic Press Ltd - Elsevier Science Ltd
Revista: Journal of Molecular and Cellular Cardiology
ISSN: 0022-2828
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Fisiología

Resumen

Abnormal intracellular Ca2+ cycling plays a key role in cardiac dysfunction, particularly during the setting of ischemia/reperfusion (I/R). During ischemia, there is an increase in cytosolic and sarcoplasmic reticulum (SR) Ca2+. At the onset of reperfusion, there is a transient and abrupt increase in cytosolic Ca2++, which occurs timely associated with reperfusion arrhythmias. However, little is known about the subcellular dynamics of Ca2+ increase during I/R, and a possible role of the SR as a mechanism underlying this increase has been previously overlooked. The aim of the present work is to test two main hypotheses: (1) An increase diastolic Ca2+ sparks frequency (cspf) constitutes a mayor substrate for the ischemia-induced diastolic Ca2+ increase; (2) an increase in cytosolic Ca2+ pro-arrhythmogenic events (Ca2+ waves), mediates the abrupt diastolic Ca2+ rise at the onset of reperfusion. We used confocal microscopy on mouse intact hearts loaded with Fluo-4. Hearts were submitted to global I/R (12/30min) to assess epicardial Ca2+ sparks in the whole heart. Intact heart sparks were faster than in isolated myocytes whereas cspf was not different. During ischemia, cspf significantly increased relative to preischemia (2.07±0.33 vs. 1.13±0.20 sp/s/100μm, n=29/34, 7 hearts). Reperfusion significantly changed Ca2+ sparks kinetics, by prolonging Ca2+ sparks rise time and decreased cspf. However, it significantly increased Ca2+ wave frequency relative to ischemia (0.71±0.14 vs. 0.38±0.06 w/s/100μm, n=32/33, 7 hearts). The results show for the first time the assessment of intact perfused heart Ca2+ sparks and provides direct evidence of increased Ca2+ sparks in ischemia that transform into Ca2+ waves during reperfusion. These waves may constitute a main trigger for reperfusion arrhythmias.
Palabras clave: Ca2+ Sparks , Intact Heart , Ischemia/Reperfusion
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/37807
DOI: http://dx.doi.org/10.1016/j.yjmcc.2014.10.011
URL: https://www.sciencedirect.com/science/article/pii/S0022282814003320
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Articulos(CIC)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Citación
Mattiazzi, Ramona Alicia; Argenziano, Mariana Alejandra; Aguilar-Sanchez, Yuriana; Mazzocchi, Gabriela; Escobar, Ariel L.; Ca2+ Sparks and Ca2+ waves are the subcellular events underlying Ca2+ overload during ischemia and reperfusion in perfused intact hearts; Academic Press Ltd - Elsevier Science Ltd; Journal of Molecular and Cellular Cardiology; 79; 2-2015; 69-78
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