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dc.contributor.author
Monerris, Melisa Jimena
dc.contributor.author
Arevalo, Fernando Javier
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Fernandez, Hector
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Zon, María Alicia
dc.contributor.author
Molina, Patricia Gabriela
dc.date.available
2018-03-01T21:06:34Z
dc.date.issued
2015-03
dc.identifier.citation
Monerris, Melisa Jimena; Arevalo, Fernando Javier; Fernandez, Hector; Zon, María Alicia; Molina, Patricia Gabriela; Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples; Elsevier Science Sa; Sensors and Actuators B: Chemical; 208; 3-2015; 525-531
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/37621
dc.description.abstract
This study describes the development of a very sensitive electrochemical immunosensor (EI) for the determination of 17β-estradiol (17β-E). The novelty of this immunosensor is that the detection of 17β-E is carried out without sample pretreatment and unlabeled neither the antigen nor the antibody. Very good results in terms of sensitivity, kinetics, and working range are obtained. The immunosensor was constructed by immobilization of the anti-17β-E monoclonal antibody (mAbE) on a gold disk electrode modified with gold nanoparticles on a cysteamine self-assembled monolayer (AuNP-cys-Au disk). Bovine serum samples were spiked with known amounts of 17β-E and incubated on mAbE-AuNP-cys-Au disk electrodes. Then, the EI was transferred to pH 5.00 citrate buffer solutions, containing horseradish peroxidase (HRP), pyrocatechol (H2Q), and H2O2 at given concentrations. The 17β-E and H2Q, both enzyme co-substrates, react with HRP. The HRP, which did not react with 17β-E, in the presence of H2O2 catalyzes the oxidation of H2Q to o-benzoquinone (Q). The back electrochemical reduction of Q to H2Q was detected on the modified gold electrode surface by square wave voltammetry. The electrochemical signal was proportional to the amount of H2Q that reacts with the enzyme, and inversely proportional to the amount of 17β-E presents in the bovine serum samples. The EI showed a linear range from 0.54 to 1.36 × 104 pg mL-1. The limit of detection (LOD) was 0.84 pg mL-1. Recovery percentages were very good, with values of 99.7, 106, and 105% for 10, 50 and 100 pg mL-1, respectively. This EI is an attractive tool for the 17β-E determination in bovine serum samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Sa
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
17Β-Estradiol
dc.subject
Enzyme Immunoassays
dc.subject
Gold Nanoparticles
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Immunosensor
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Development of a very sensitive electrochemical immunosensor for the determination of 17β-estradiol in bovine serum samples
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-27T14:17:05Z
dc.journal.volume
208
dc.journal.pagination
525-531
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Monerris, Melisa Jimena. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Molina, Patricia Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2014.11.048
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092540051401418X
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