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dc.contributor.author
Colombo, Lucas
dc.contributor.author
Baruzzi, Ana Maria
dc.contributor.author
Garay, Fernando Sebastian
dc.date.available
2018-02-27T21:42:41Z
dc.date.issued
2015-01
dc.identifier.citation
Colombo, Lucas; Baruzzi, Ana Maria; Garay, Fernando Sebastian; Analysis and optimization of a hydrogel matrix for the development of a sandwich-type glucose biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 211; 1-2015; 125-130
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/37369
dc.description.abstract
The development of a sandwich-type biosensor for glucose quantification is presented. This work is focused on the optimization of the enzymatic matrix of the biosensor. The best performance was found for an enzymatic matrix composed by 30% w/w mucin, 70% w/w albumin, 1.35 U glucose oxidase (GOX) per sensor, and glutaraldehyde diluted to 3%. The crosslinking with glutaraldehyde transforms this mixture into a hydrogel that is entrapped between two membranes of polycarbonate. The selected sandwich-type biosensor showed very good response time, sensitivity, stability, and sensor-to-sensor reproducibility. According to the results presented in this manuscript, a biosensor prepared with very high amount of enzyme would not necessarily increase the analytical signal. Simulated curves are compared with experimental data to explain the dependence of sensitivity on the concentration of enzyme. In addition, this kind of comparison represents a quite simple way to estimate the value of vmax≈0.13 M s-1 from the amperometric response of a sensor prepared with 1.34 U of GOX. Considering that sandwich-type biosensors are commonly assembled as part of devices where the sample is diluted with buffer, the more than 3 orders of magnitude of linear behavior of this sensor would ensure the possibility for assessing any sample.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Sa
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Electrochemical Biosensor
dc.subject
Glucose
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Hydrogel
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Mathematical Model
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Sandwich-Type Biosensor
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Analysis and optimization of a hydrogel matrix for the development of a sandwich-type glucose biosensor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-27T14:17:08Z
dc.journal.volume
211
dc.journal.pagination
125-130
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Colombo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2015.01.063
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092540051500088X
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