Artículo
Effect of several PEO-PPO amphiphiles on bax, bcl-2, and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients
Fecha de publicación:
03/2016
Editorial:
Wiley
Revista:
Biotechnology and Applied Biochemistry
ISSN:
0885-4513
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis. Aim: This article aims to investigate the effect of poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT-PCR for bax, bcl-2, and human telomerase reverse transcriptase (hTERT). Results: PEO-PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines. Conclusions: PEO-PPO block copolymers - considered safe for human use - can drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.
Palabras clave:
Bax
,
Bcl-2
,
Htert
,
Multiplex Rt-Pcr
,
Poloxamers
,
Poloxamines
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Identificadores
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Castronuovo, Cynthia Celeste; Cuestas, María Luján; Oubiña, Jose Raul; Mathet, Veronica Lidia; Effect of several PEO-PPO amphiphiles on bax, bcl-2, and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients; Wiley; Biotechnology and Applied Biochemistry; 63; 2; 3-2016; 273-280
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