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dc.contributor.author
Bisoffi, Zeno  
dc.contributor.author
Buonfrate, Dora  
dc.contributor.author
Sequi, Marco  
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Mejia, Rojelio  
dc.contributor.author
Cimino, Rubén Oscar  
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Krolewiecki, Alejandro Javier  
dc.contributor.author
Albonico, Marco  
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Gobbo, Maria  
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Bonafini, Stefania  
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Angheben, Andrea  
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Requena-Mendez, Ana  
dc.contributor.author
Muñoz, José  
dc.contributor.author
Nutman, Thomas B.  
dc.date.available
2018-02-26T18:12:41Z  
dc.date.issued
2014-01  
dc.identifier.citation
Bisoffi, Zeno; Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Rubén Oscar; et al.; Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 1-8; e2640  
dc.identifier.issn
1935-2735  
dc.identifier.uri
http://hdl.handle.net/11336/37122  
dc.description.abstract
Background:The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard.Methods:The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests).Results:According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity.Conclusions:NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Public Library of Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Strongyloides Stercoralis  
dc.subject
Serology  
dc.subject
Diagnostics  
dc.subject
Lips  
dc.subject.classification
Medicina Tropical  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-09-01T18:04:18Z  
dc.journal.volume
8  
dc.journal.number
1  
dc.journal.pagination
1-8; e2640  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
San Francisco  
dc.description.fil
Fil: Bisoffi, Zeno. Sacro Cuore Hospital; Italia  
dc.description.fil
Fil: Buonfrate, Dora. Sacro Cuore Hospital; Italia  
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Fil: Sequi, Marco. Istituto Di Ricerche Farmacologiche Mario Negri; Italia  
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Fil: Mejia, Rojelio. National Institute Of Allergy And Infectious Diseases; Estados Unidos  
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Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Albonico, Marco. Sacro Cuore Hospital; Italia  
dc.description.fil
Fil: Gobbo, Maria. Sacro Cuore Hospital; Italia  
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Fil: Bonafini, Stefania. Sacro Cuore Hospital; Italia  
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Fil: Angheben, Andrea. Sacro Cuore Hospital; Italia  
dc.description.fil
Fil: Requena-Mendez, Ana. Universidad de Barcelona; España  
dc.description.fil
Fil: Muñoz, José. Universidad de Barcelona; España  
dc.description.fil
Fil: Nutman, Thomas B.. National Institute Of Allergy And Infectious Diseases; Estados Unidos  
dc.journal.title
Neglected Tropical Diseases  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0002640  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002640