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dc.contributor.author
Lanfranconi, Mariana Patricia
dc.contributor.author
Alvarez, Hector Manuel
dc.date.available
2018-02-21T21:26:10Z
dc.date.issued
2017-10-14
dc.identifier.citation
Lanfranconi, Mariana Patricia; Alvarez, Hector Manuel; Rewiring neutral lipids production for the de novo synthesis of wax esters in Rhodococcus opacus PD630; Elsevier Science; Journal of Biotechnology; 260; 14-10-2017; 67-73
dc.identifier.issn
0168-1656
dc.identifier.uri
http://hdl.handle.net/11336/36944
dc.description.abstract
Rhodococcus opacus PD630 accumulates significant amounts of triacylglycerols (TAG), but is not able to de novo synthesize wax esters (WE) from structural unrelated carbon sources, such as gluconate. In this study, strain PD630 was engineered to produce WE by heterologous expression of maqu_2220 gene, which encodes a fatty acyl-CoA reductase for the production of fatty alcohols in Marinobacter hydrocarbonoclasticus. Recombinant cells produced ca. 46% of WE and 54% of TAG (of total WE + TAG) from gluconate compared with the wild type, which produced 100% of TAG. Cell growth was not affected by the heterologous expression of MAQU_2220. Several saturated and monounsaturated WE species were produced by cells, with C18:C16, C16:C16 and C16:C18 as main species. The fatty acid composition of WE fraction in PD630maqu_2220 was enriched with C16:0, C18:0, whereas C16:0, C18:0 and C18:1 predominated in the TAG fraction. Significant amounts of WE and TAG were accumulated by PD630maqu_2220 from whey, an inexpensive waste material from dairy industries, without affecting cell biomass production. This is the first report on WE synthesis by R. opacus from gluconate, which demonstrates that lipid metabolism of this bacterium is flexible enough to assimilate heterologous components for the production of new lipid derivatives with industrial interest.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fatty Acyl-Coa Reductase
dc.subject
Rhodococcus Opacus
dc.subject
Triacylglycerols
dc.subject
Wax Esters
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Rewiring neutral lipids production for the de novo synthesis of wax esters in Rhodococcus opacus PD630
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-20T17:07:14Z
dc.journal.volume
260
dc.journal.pagination
67-73
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Lanfranconi, Mariana Patricia. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
dc.description.fil
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia "san Juan Bosco". Instituto de Biociencias de la Patagonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biociencias de la Patagonia; Argentina
dc.journal.title
Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0168165617316498
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jbiotec.2017.09.009
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