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dc.contributor.author
Juarez, Antonio
dc.contributor.author
Villa Guerrero, Juan Angel
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Lanza, Val F.
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Lázaro, Beatriz
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de la Cruz, Fernando
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Alvarez, Hector Manuel
dc.contributor.author
Moncalián, Gabriel
dc.date.available
2018-02-21T21:21:30Z
dc.date.issued
2017-02-27
dc.identifier.citation
Juarez, Antonio; Villa Guerrero, Juan Angel; Lanza, Val F.; Lázaro, Beatriz; de la Cruz, Fernando; et al.; Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1; BioMed Central; Microbial Cell Factories; 16; 35; 27-2-2017; 1-12
dc.identifier.issn
1475-2859
dc.identifier.uri
http://hdl.handle.net/11336/36941
dc.description.abstract
Background: Rhodococcus jostii RHA1 and other actinobacteria accumulate triglycerides (TAG) under nutrient starvation. This property has an important biotechnological potential in the production of sustainable oils. Results: To gain insight into the metabolic pathways involved in TAG accumulation, we analysed the transcriptome of R jostii RHA1 under nutrient-limiting conditions. We correlate these physiological conditions with significant changes in cell physiology. The main consequence was a global switch from catabolic to anabolic pathways. Interestingly, the Entner-Doudoroff (ED) pathway was upregulated in detriment of the glycolysis or pentose phosphate pathways. ED induction was independent of the carbon source (either gluconate or glucose). Some of the diacylglycerol acyltransferase genes involved in the last step of the Kennedy pathway were also upregulated. A common feature of the promoter region of most upregulated genes was the presence of a consensus binding sequence for the cAMP-dependent CRP regulator. Conclusion: This is the first experimental observation of an ED shift under nutrient starvation conditions. Knowledge of this switch could help in the design of metabolomic approaches to optimize carbon derivation for single cell oil production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Crp
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Entner-Doudoroff Pathway
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Nutrient Starvation
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Rhodococcus
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Rna-Seq
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Triacylglycerol
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Nutrient starvation leading to triglyceride accumulation activates the Entner Doudoroff pathway in Rhodococcus jostii RHA1
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-20T17:06:48Z
dc.journal.volume
16
dc.journal.number
35
dc.journal.pagination
1-12
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Juarez, Antonio. Institut de Bioenginyeria de Catalunya; España
dc.description.fil
Fil: Villa Guerrero, Juan Angel. Universidad de Cantabria; España
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Fil: Lanza, Val F.. Universidad de Cantabria; España
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Fil: Lázaro, Beatriz. Universidad de Cantabria; España
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Fil: de la Cruz, Fernando. Universidad de Cantabria; España
dc.description.fil
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia ; Argentina
dc.description.fil
Fil: Moncalián, Gabriel. Universidad de Cantabria; España
dc.journal.title
Microbial Cell Factories
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12934-017-0651-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0651-7
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