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dc.contributor.author
Lupo, Maela
dc.contributor.author
Brance, María Lorena
dc.contributor.author
Fina, Brenda Lorena
dc.contributor.author
Brun, Lucas Ricardo Martín
dc.contributor.author
Rigalli, Alfredo
dc.date.available
2018-02-20T20:18:16Z
dc.date.issued
2015-01
dc.identifier.citation
Lupo, Maela; Brance, María Lorena; Fina, Brenda Lorena; Brun, Lucas Ricardo Martín; Rigalli, Alfredo; Methodology developed for the simultaneous measurement of bone formation and bone resorption in rats based on the pharmacokinetics of fluoride; Springer Tokyo; Journal of bone and mineral metabolism; 33; 1; 1-2015; 16-22
dc.identifier.issn
0914-8779
dc.identifier.uri
http://hdl.handle.net/11336/36842
dc.description.abstract
This paper describes a novel methodology for the simultaneous estimation of bone formation (BF) and resorption (BR) in rats using fluoride as a nonradioactive bone-seeker ion. The pharmacokinetics of flouride have been extensively studied in rats; its constants have all been characterized. This knowledge was the cornerstone for the underlying mathematical model that we used to measure bone fluoride uptake and elimination rate after a dose of fluoride. Bone resorption and formation were estimated by bone fluoride uptake and elimination rate, respectively. ROC analysis showed that sensitivity, specificity and area under the ROC curve were not different from deoxypiridinoline and bone alkaline phosphatase, well-known bone markers. Sprague-Dawley rats with modified bone remodelling (ovariectomy, hyper, and hypocalcic diet, antiresorptive treatment) were used to validate the values obtained with this methodology. The results of BF and BR obtained with this technique were as expected for each biological model. Although the method should be performed under general anesthesia, it has several advantages: simultaneous measurement of BR and BF, low cost, and the use of compounds with no expiration date.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Tokyo
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bone Remodelling
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Mathematical Model
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Flouride
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Bone Markers
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Methodology developed for the simultaneous measurement of bone formation and bone resorption in rats based on the pharmacokinetics of fluoride
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-02-08T17:54:43Z
dc.identifier.eissn
1435-5604
dc.journal.volume
33
dc.journal.number
1
dc.journal.pagination
16-22
dc.journal.pais
Japón
dc.journal.ciudad
Tokyo
dc.description.fil
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Brance, María Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
dc.description.fil
Fil: Fina, Brenda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
dc.description.fil
Fil: Brun, Lucas Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
dc.description.fil
Fil: Rigalli, Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
dc.journal.title
Journal of bone and mineral metabolism
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00774-013-0557-3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00774-013-0557-3
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