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dc.contributor.author
Briceño, G.
dc.contributor.author
Schalchli, H.
dc.contributor.author
Rubilar, O.
dc.contributor.author
Tortella, G.R.
dc.contributor.author
Mutis, A.
dc.contributor.author
Benimeli, Claudia Susana
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dc.contributor.author
Palma, G.
dc.contributor.author
Diez, M.C.
dc.date.available
2018-02-19T20:33:09Z
dc.date.issued
2016-08
dc.identifier.citation
Briceño, G.; Schalchli, H.; Rubilar, O.; Tortella, G.R.; Mutis, A.; et al.; Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture; Pergamon-Elsevier Science Ltd.; Chemosphere; 156; 8-2016; 195-203
dc.identifier.issn
0045-6535
dc.identifier.uri
http://hdl.handle.net/11336/36775
dc.description.abstract
Actinobacteria identified as Streptomyces spp. were evaluated for their ability to remove diazinon as the only carbon source from a liquid medium. Single cultures of Streptomyces strains were exposed to diazinon at a concentration of 50 mg L-1. After 96 h incubation, six of the eight cultures grew and five strains showed an increase in their total protein concentrations and changes in their protein profile. Up to 32% of the diazinon was removed by the single Streptomyces cultures. A compatibility assay showed that the different Streptomyces species were not antagonistic. Twenty-six mixed cultures were then prepared. Diazinon removal was increased when mixed cultures were used, and maximum diazinon removal of 62% was observed when the Streptomyces spp. strains AC5, AC9, GA11 and ISP13 were mixed; this was defined as the selected mixed culture (SMC). Diazinon removal was positively influenced by the addition of glucose into the liquid medium. Our study showed a diazinon degradation rate of 0.025 h-1, half-life of 28 h-1 and 2-isopropyl-6-methyl-4-pyrimidinol (IMHP) production of 0.143 mg L h-1. Rapid diazinon hydrolysis to IMHP was associated with a decrease in the pH of the medium as a consequence of microbial glucose metabolism and organic acid exudation. Moreover, the SMC of Streptomyces was able to remove IMHP. This work constitutes a new, if not the only, report on diazinon degradation by mixed cultures of Streptomyces spp. Given the high levels of diazinon removal, the SMC formed by four Streptomyces strains has the potential to be used to treat the diazinon present in environmental matrices.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd.
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Mixed Cultures
dc.subject
Actinobacteria
dc.subject
Degradation
dc.subject
Diazinon
dc.subject.classification
Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
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dc.subject.classification
Biotecnología del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-04T18:00:01Z
dc.journal.volume
156
dc.journal.pagination
195-203
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Briceño, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Schalchli, H.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Rubilar, O.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Tortella, G.R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Mutis, A.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
dc.description.fil
Fil: Palma, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.description.fil
Fil: Diez, M.C.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
dc.journal.title
Chemosphere
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chemosphere.2016.04.118
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